IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v49y2004i3id4285-cjas.html
   My bibliography  Save this article

In vitro aging of porcine oocytes

Author

Listed:
  • I. Petrová

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • M. Sedmíková

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • E. Chmelíková

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • D. Švestková

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • R. Rajmon

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

Abstract

Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, most of them remained at the stage of metaphase II (98%). Then many oocytes underwent the spontaneous parthenogenetic activation. The portion of activated oocytes reached its peak after 2 or 3 days of aging in vitro (39 or 45%). The portion of fragmented oocytes peaked at the same time (28%). During subsequent aging in vitro (i.e. day 4 or 5 of aging), the portion of lysed oocytes significantly increased (30 or 37%). The highest portion of spontaneously activated parthenogenetic embryos at a pronuclear stage (35%) was observed during the 2nd day of aging in vitro. These pronuclear embryos had mainly one polar body with two pronuclei (47% of all pronuclear embryos) or two polar bodies with one pronucleus (38% of all pronuclear embryos). During the 3rd and 5th day of in vitro aging, there was a significant increase in the portion of parthenogenetic embryos cleaved to the 2-cell or 3-cell stage. When considering the prolonged in vitro culture of porcine oocyte, only the first day of aging should be taken into account, since beyond this time significant changes, i.e. parthenogenesis, fragmentation or lysis, occurred in oocytes under in vitro conditions.

Suggested Citation

  • I. Petrová & M. Sedmíková & E. Chmelíková & D. Švestková & R. Rajmon, 2004. "In vitro aging of porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 49(3), pages 93-98.
    • Handle: RePEc:caa:jnlcjs:v:49:y:2004:i:3:id:4285-cjas
    DOI: 10.17221/4285-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/4285-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/4285-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/4285-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. J. Petr & E. Chmelíková & L. Tůmová & M. Ješeta, 2007. "The role of nitric oxide in parthenogenetic activation of pig oocytes: A review," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(11), pages 363-377.
    2. J. Nevoral & T. Krejčová & J. Petr & P. Melicharová & A. Vyskočilová & M. Dvořáková & I. Weingartová & E. Chmelíková & L. Tůmová & K. Hošková & V. Kučerová-Chrpová & M. Sedmíková, 2013. "The role of nitric oxide synthase isoforms in aged porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(10), pages 453-459.
    3. M. Sedmíková & J. Petr & A. Dörflerová & J. Nevoral & B. Novotná & T. Krejčová & E. Chmelíková & L. Tůmová, 2013. "Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(12), pages 535-545.
    4. J. Petr & E. Chmelíková & A. Dörflerová & M. Ješeta & Z. Kuthanová, 2007. "Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(12), pages 415-422.
    5. I. Petrová & R. Rajmon & M. Sedmíková & Z. Kuthanová & F. Jílek & J. Rozinek, 2005. "Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(7), pages 300-310.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:49:y:2004:i:3:id:4285-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.