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In vitro aging of porcine oocytes

Author

Listed:
  • I. Petrová

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • M. Sedmíková

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • E. Chmelíková

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • D. Švestková

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

  • R. Rajmon

    (, M. S 2, E. C 2, D. Š 2, R. R 2 1Grammar School, Říčany near Prague, Czech Republic)

Abstract

Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, most of them remained at the stage of metaphase II (98%). Then many oocytes underwent the spontaneous parthenogenetic activation. The portion of activated oocytes reached its peak after 2 or 3 days of aging in vitro (39 or 45%). The portion of fragmented oocytes peaked at the same time (28%). During subsequent aging in vitro (i.e. day 4 or 5 of aging), the portion of lysed oocytes significantly increased (30 or 37%). The highest portion of spontaneously activated parthenogenetic embryos at a pronuclear stage (35%) was observed during the 2nd day of aging in vitro. These pronuclear embryos had mainly one polar body with two pronuclei (47% of all pronuclear embryos) or two polar bodies with one pronucleus (38% of all pronuclear embryos). During the 3rd and 5th day of in vitro aging, there was a significant increase in the portion of parthenogenetic embryos cleaved to the 2-cell or 3-cell stage. When considering the prolonged in vitro culture of porcine oocyte, only the first day of aging should be taken into account, since beyond this time significant changes, i.e. parthenogenesis, fragmentation or lysis, occurred in oocytes under in vitro conditions.

Suggested Citation

  • I. Petrová & M. Sedmíková & E. Chmelíková & D. Švestková & R. Rajmon, 2004. "In vitro aging of porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 49(3), pages 93-98.
    • Handle: RePEc:caa:jnlcjs:v:49:y:2004:i:3:id:4285-cjas
    DOI: 10.17221/4285-CJAS
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    Citations

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    Cited by:

    1. J. Petr & E. Chmelíková & L. Tůmová & M. Ješeta, 2007. "The role of nitric oxide in parthenogenetic activation of pig oocytes: A review," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(11), pages 363-377.
    2. M. Sedmíková & J. Petr & A. Dörflerová & J. Nevoral & B. Novotná & T. Krejčová & E. Chmelíková & L. Tůmová, 2013. "Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(12), pages 535-545.
    3. J. Petr & E. Chmelíková & A. Dörflerová & M. Ješeta & Z. Kuthanová, 2007. "Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(12), pages 415-422.
    4. J. Nevoral & T. Krejčová & J. Petr & P. Melicharová & A. Vyskočilová & M. Dvořáková & I. Weingartová & E. Chmelíková & L. Tůmová & K. Hošková & V. Kučerová-Chrpová & M. Sedmíková, 2013. "The role of nitric oxide synthase isoforms in aged porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(10), pages 453-459.
    5. I. Petrová & R. Rajmon & M. Sedmíková & Z. Kuthanová & F. Jílek & J. Rozinek, 2005. "Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(7), pages 300-310.

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