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Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor

Author

Listed:
  • J. Petr

    (Institute of Animal Science, Prague-Uhříněves, Czech Republic)

  • E. Chmelíková

    (Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic)

  • A. Dörflerová

    (Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic)

  • M. Ješeta

    (Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic)

  • Z. Kuthanová

    (Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic)

Abstract

Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50μM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours). Protein kinase C (PKC) inhibitors, bisindolylmaleimide I or rottlerin, are able to inhibit parthenogenetic activation induced by calcium ionophore. The rate of activated oocytes decreased from 69% to 2% (P < 0.05) under the effect of bisindolylmaleimide I at a concentration of 0 or 20nM, respectively. The activation rate decreased from 68% to 0% (P < 0.05) under the influence of 0 or 20μM rottlerin, respectively. PKC inhibitors Go6976 or hispidin had no effect on the oocyte activation using calcium ionophore or on oocytes activated by a nitric oxide donor. The activation of oocytes by a nitric oxide donor is not significantly influenced even under the effects of bisindolylmaleimide I or rottlerin. Based on these data we can conclude that the oocyte activation induced by calcium ionophore depends on PKC, especially on PKC-δ. On the other hand, the oocyte activation induced by nitric oxide is independent of the tested isotypes of PKC.

Suggested Citation

  • J. Petr & E. Chmelíková & A. Dörflerová & M. Ješeta & Z. Kuthanová, 2007. "Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(12), pages 415-422.
    • Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:12:id:2336-cjas
    DOI: 10.17221/2336-CJAS
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    References listed on IDEAS

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    1. A. Wierzchos, 2006. "Parthenogenetic development of rabbit oocytes after electrical stimulation," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(9), pages 400-405.
    2. I. Petrová & R. Rajmon & M. Sedmíková & Z. Kuthanová & F. Jílek & J. Rozinek, 2005. "Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(7), pages 300-310.
    3. I. Petrová & M. Sedmíková & E. Chmelíková & D. Švestková & R. Rajmon, 2004. "In vitro aging of porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 49(3), pages 93-98.
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    Cited by:

    1. J. Petr & E. Chmelíková & L. Tůmová & M. Ješeta, 2007. "The role of nitric oxide in parthenogenetic activation of pig oocytes: A review," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(11), pages 363-377.
    2. E. Chmelíková & M. Sedmíková & J. Petr & T. Kott & V. Lánská & L. Tůmová & H. Tichovská & M. Ješeta, 2009. "Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(4), pages 137-149.
    3. T. Krejčová & J. Petr & M. Krejčová & K. Kheilová, 2009. "Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(7), pages 293-306.

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