IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0228958.html
   My bibliography  Save this article

Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea

Author

Listed:
  • Kuo-Tung Huang
  • Yung-Che Chen
  • Chia-Cheng Tseng
  • Huang-Chih Chang
  • Mao-Chang Su
  • Ting-Ya Wang
  • Yong-Yong Lin
  • Yi-Xin Zheng
  • Jen-Chieh Chang
  • Chien-Hung Chin
  • Chang-Chun Hsiao
  • Meng-Chih Lin

Abstract

Obstructive sleep apnea (OSA) is a syndrome leading to chronic intermittent hypoxia, and the up-regulation of toll-like receptors (TLR) 2 and 6 on peripheral blood cells has been reported. We hypothesized that DNA methylation in TLR2 and TLR6 genes may play a role in the development of OSA and its excessive daytime sleepiness (EDS) phenotype. DNA methylation over 28 cytosine-phosphate-guanine (CpG) sites of the TLR2 promoter region and 3 CpG sites of the TLR6 gene body, and their protein expressions were measured by using pyrosequencing and ELISA methods in 18 heathy subjects (HS) and 58 patients with severe OSA (divided into 18 non-EDS and 40 EDS group). Patients with severe OSA had higher DNA methylation levels over five CpG sites (#1, #2, #3, #25 and #28) and lower DNA methylation levels over CpG site #18 of the TLR2 promoter region, higher DNA methylation levels over two CpG sites (#1 and #3) of the TLR6 gene body, and higher protein expressions of TLR6 than HS. The CpG site #2 of the TLR6 gene body was hypermethylated in severe OSA patients with EDS. Both DNA methylation levels over CpG site #1 of the TLR6 gene body and protein expressions of TLR6 were reduced after more than 6 months of nasal CPAP treatment in seven selected patients. Aberrant DNA methylation of the TLR2 promoter region and TLR6 gene body are associated with the consequence of severe OSA and its EDS phenotype.

Suggested Citation

  • Kuo-Tung Huang & Yung-Che Chen & Chia-Cheng Tseng & Huang-Chih Chang & Mao-Chang Su & Ting-Ya Wang & Yong-Yong Lin & Yi-Xin Zheng & Jen-Chieh Chang & Chien-Hung Chin & Chang-Chun Hsiao & Meng-Chih Lin, 2020. "Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea," PLOS ONE, Public Library of Science, vol. 15(2), pages 1-16, February.
  • Handle: RePEc:plo:pone00:0228958
    DOI: 10.1371/journal.pone.0228958
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228958
    Download Restriction: no

    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0228958&type=printable
    Download Restriction: no

    File URL: https://libkey.io/10.1371/journal.pone.0228958?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Sergio Garbarino & Paolo Durando & Ottavia Guglielmi & Guglielmo Dini & Francesca Bersi & Stefania Fornarino & Alessandra Toletone & Carlo Chiorri & Nicola Magnavita, 2016. "Sleep Apnea, Sleep Debt and Daytime Sleepiness Are Independently Associated with Road Accidents. A Cross-Sectional Study on Truck Drivers," PLOS ONE, Public Library of Science, vol. 11(11), pages 1-12, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Sergio Garbarino & Ottavia Guglielmi & Matteo Puntoni & Nicola Luigi Bragazzi & Nicola Magnavita, 2019. "Sleep Quality among Police Officers: Implications and Insights from a Systematic Review and Meta-Analysis of the Literature," IJERPH, MDPI, vol. 16(5), pages 1-15, March.
    2. Nicola Magnavita & Sergio Garbarino, 2017. "Sleep, Health and Wellness at Work: A Scoping Review," IJERPH, MDPI, vol. 14(11), pages 1-18, November.
    3. Anna Tzortzi & Melpo Kapetanstrataki & Vaso Evangelopoulou & Panagiotis Behrakis, 2021. "Driving Behavior That Limits Concentration: A Nationwide Survey in Greece," IJERPH, MDPI, vol. 18(8), pages 1-16, April.
    4. Sergio Garbarino & Ottavia Guglielmi & Walter G Sannita & Nicola Magnavita & Paola Lanteri, 2018. "Sleep and Mental Health in Truck Drivers: Descriptive Review of the Current Evidence and Proposal of Strategies for Primary Prevention," IJERPH, MDPI, vol. 15(9), pages 1-13, August.
    5. Nicola Luigi Bragazzi & Guglielmo Dini & Alessandra Toletone & Alborz Rahmani & Alfredo Montecucco & Emanuela Massa & Alessia Manca & Ottavia Guglielmi & Sergio Garbarino & Nicoletta Debarbieri & Paol, 2018. "Patterns of Harmful Alcohol Consumption among Truck Drivers: Implications for Occupational Health and Work Safety from a Systematic Review and Meta-Analysis," IJERPH, MDPI, vol. 15(6), pages 1-22, May.
    6. Fernanda Lise & Mona Shattell & Raquel Pötter Garcia & Kethelyn Costa Rodrigues & Wilson Teixeira de Ávila & Flávia Lise Garcia & Eda Schwartz, 2024. "Long-Haul Truck Drivers’ Perceptions of Truck Stops and Rest Areas: Focusing on Health and Wellness," IJERPH, MDPI, vol. 21(9), pages 1-13, September.
    7. Sergio Garbarino & Giovanni Tripepi & Nicola Magnavita, 2020. "Sleep Health Promotion in the Workplace," IJERPH, MDPI, vol. 17(21), pages 1-14, October.
    8. Ari Min & Minkyung Kang & Hye Chong Hong, 2021. "Sickness Presenteeism in Shift and Non-Shift Nurses: Using the Fifth Korean Working Conditions Survey," IJERPH, MDPI, vol. 18(6), pages 1-12, March.
    9. Xinyong Zhang & Chaoyue Zhao & Zhaoxiang Niu & Shike Xu & Dawei Wang, 2021. "Job Insecurity and Safety Behaviour: The Mediating Role of Insomnia and Work Engagement," IJERPH, MDPI, vol. 18(2), pages 1-15, January.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0228958. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.