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Comprehensive Mapping of Regional Expression of the Clock Protein PERIOD2 in Rat Forebrain across the 24-h Day

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  • Valerie L Harbour
  • Yuval Weigl
  • Barry Robinson
  • Shimon Amir

Abstract

In mammals, a light-entrainable clock located in the suprachiasmatic nucleus (SCN) regulates circadian rhythms by synchronizing oscillators throughout the brain and body. Notably, the nature of the relation between the SCN clock and subordinate oscillators in the rest of the brain is not well defined. We performed a high temporal resolution analysis of the expression of the circadian clock protein PERIOD2 (PER2) in the rat forebrain to characterize the distribution, amplitude and phase of PER2 rhythms across different regions. Eighty-four LEW/Crl male rats were entrained to a 12-h: 12-h light/dark cycle, and subsequently perfused every 30 min across the 24-h day for a total of 48 time-points. PER2 expression was assessed with immunohistochemistry and analyzed using automated cell counts. We report the presence of PER2 expression in 20 forebrain areas important for a wide range of motivated and appetitive behaviors including the SCN, bed nucleus, and several regions of the amygdala, hippocampus, striatum, and cortex. Eighteen areas displayed significant PER2 rhythms, which peaked at different times of day. Our data demonstrate a previously uncharacterized regional distribution of rhythms of a clock protein expression in the brain that provides a sound basis for future studies of circadian clock function in animal models of disease.

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  • Valerie L Harbour & Yuval Weigl & Barry Robinson & Shimon Amir, 2013. "Comprehensive Mapping of Regional Expression of the Clock Protein PERIOD2 in Rat Forebrain across the 24-h Day," PLOS ONE, Public Library of Science, vol. 8(10), pages 1-14, October.
    • Handle: RePEc:plo:pone00:0076391
    DOI: 10.1371/journal.pone.0076391
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    References listed on IDEAS

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    1. Binhai Zheng & David W. Larkin & Urs Albrecht & Zhong Sheng Sun & Marijke Sage & Gregor Eichele & Cheng Chi Lee & Allan Bradley, 1999. "The mPer2 gene encodes a functional component of the mammalian circadian clock," Nature, Nature, vol. 400(6740), pages 169-173, July.
    2. Steven M. Reppert & David R. Weaver, 2002. "Coordination of circadian timing in mammals," Nature, Nature, vol. 418(6901), pages 935-941, August.
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    1. Sherin Al-Safadi & Aya Al-Safadi & Marie Branchaud & Spencer Rutherford & Arun Dayanandan & Barry Robinson & Shimon Amir, 2014. "Stress-Induced Changes in the Expression of the Clock Protein PERIOD1 in the Rat Limbic Forebrain and Hypothalamus: Role of Stress Type, Time of Day, and Predictability," PLOS ONE, Public Library of Science, vol. 9(10), pages 1-16, October.
    2. Valerie L Harbour & Yuval Weigl & Barry Robinson & Shimon Amir, 2014. "Phase Differences in Expression of Circadian Clock Genes in the Central Nucleus of the Amygdala, Dentate Gyrus, and Suprachiasmatic Nucleus in the Rat," PLOS ONE, Public Library of Science, vol. 9(7), pages 1-9, July.

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