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Long-Term Sphere Culture Cannot Maintain a High Ratio of Cancer Stem Cells: A Mathematical Model and Experiment

Author

Listed:
  • Tang Peng
  • Ma Qinghua
  • Tang Zhenning
  • Wang Kaifa
  • Jiang Jun

Abstract

Acquiring abundant and high-purity cancer stem cells (CSCs) is an important prerequisite for CSC research. At present, researchers usually gain high-purity CSCs through flow cytometry sorting and expand them by short-term sphere culture. However, it is still uncertain whether we can amplify high-purity CSCs through long-term sphere culture. We have proposed a mathematical model using ordinary differential equations to derive the continuous variation of the CSC ratio in long-term sphere culture and estimated the model parameters based on a long-term sphere culture of MCF-7 stem cells. We found that the CSC ratio in long-term sphere culture presented as gradually decreased drift and might be stable at a lower level. Furthermore, we found that fitted model parameters could explain the main growth pattern of CSCs and differentiated cancer cells in long-term sphere culture.

Suggested Citation

  • Tang Peng & Ma Qinghua & Tang Zhenning & Wang Kaifa & Jiang Jun, 2011. "Long-Term Sphere Culture Cannot Maintain a High Ratio of Cancer Stem Cells: A Mathematical Model and Experiment," PLOS ONE, Public Library of Science, vol. 6(11), pages 1-6, November.
    • Handle: RePEc:plo:pone00:0025518
    DOI: 10.1371/journal.pone.0025518
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    References listed on IDEAS

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    2. Sean J. Morrison & Judith Kimble, 2006. "Asymmetric and symmetric stem-cell divisions in development and cancer," Nature, Nature, vol. 441(7097), pages 1068-1074, June.
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