Standardising visual control devices for Tsetse: East and Central African Savannah species Glossina swynnertoni, Glossina morsitans centralis and Glossina pallidipes

Background: This study focused on the savannah tsetse species Glossina swynnertoni and G. morsitans centralis, both efficient vectors of human and animal trypanosomiasis in, respectively, East and Central Africa. The aim was to develop long-lasting, practical and cost-effective visually attractive devices that induce the strongest landing responses in these two species for use as insecticide-impregnated tools in population suppression. Methods and findings: Trials were conducted in different seasons and years in Tanzania (G. swynnertoni) and in Angola and the Democratic Republic of the Congo (DRC, G. m. centralis) to measure the performance of traps (pyramidal and epsilon) and targets of different sizes, shapes and colours, with and without chemical baits, at different population densities and under different environmental conditions. Adhesive film was used to catch flies landing on devices at the remote locations to compare tsetse-landing efficiencies. Landing rates by G. m. centralis in both Angola and the DRC were highest on blue-black 1 m2 oblong and 0.5 m2 square and oblong targets but were not significantly different from landings on the pyramidal trap. Landings by G. swynnertoni on 0.5 m2 blue-black oblong targets were likewise not significantly lower than on equivalent 1 m2 square targets. The length of target horizontal edge was closely correlated with landing rate. Blue-black 0.5 m2 targets performed better than equivalents in all-blue for both G. swynnertoni and G. m. centralis, although not consistently. Baiting with chemicals increased the proportion of G. m. centralis entering pyramidal traps. Conclusions: This study confirms earlier findings on G. swynnertoni that smaller visual targets, down to 0.5 m2, would be as efficient as using 1 m2 targets for population management of this species. This is also the case for G. m. centralis. An insecticide-impregnated pyramidal trap would also constitute an effective control device for G. m. centralis. Author summary: Glossina swynnertoni is restricted to open savannah in northwestern Tanzania and southwestern Kenya whereas G. morsitans centralis has a much wider distribution from western Tanzania/southern Uganda westwards through Zambia and southeast of the Democratic Republic of the Congo (DRC) to Angola. Both are savannah tsetse and are efficient vectors of human and animal trypanosomiasis. In comparison to other tsetse species, relatively little work has been done to test the efficacy of traps and targets for controlling G. swynnertoni and G. m. centralis. To determine the most visually-attractive and practical objects we conducted field tests with devices of various shapes, sizes and colours in Tanzania, DRC and Angola in different years, seasons, environmental conditions and at different population densities. The strongest landing responses were on 0.5 m2 horizontal rectangular targets with respect to ground that had both black and phthalogen blue elements with fly landing rates not significantly lower than on equivalent 1 m2 targets used till now for both species. The pyramidal trap proved efficient as a landing stimulus as targets of either size for G. m. centralis. Insecticide-impregnated blue-black 0.5 m2 cloth targets show promise as cost-effective devices for management of G. swynnertoni and G. m. centralis populations.