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A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport

Author

Listed:
  • Ricardo Celestino
  • Morkos A Henen
  • José B Gama
  • Cátia Carvalho
  • Maxwell McCabe
  • Daniel J Barbosa
  • Alexandra Born
  • Parker J Nichols
  • Ana X Carvalho
  • Reto Gassmann
  • Beat Vögeli

Abstract

All animal cells use the motor cytoplasmic dynein 1 (dynein) to transport diverse cargo toward microtubule minus ends and to organize and position microtubule arrays such as the mitotic spindle. Cargo-specific adaptors engage with dynein to recruit and activate the motor, but the molecular mechanisms remain incompletely understood. Here, we use structural and dynamic nuclear magnetic resonance (NMR) analysis to demonstrate that the C-terminal region of human dynein light intermediate chain 1 (LIC1) is intrinsically disordered and contains two short conserved segments with helical propensity. NMR titration experiments reveal that the first helical segment (helix 1) constitutes the main interaction site for the adaptors Spindly (SPDL1), bicaudal D homolog 2 (BICD2), and Hook homolog 3 (HOOK3). In vitro binding assays show that helix 1, but not helix 2, is essential in both LIC1 and LIC2 for binding to SPDL1, BICD2, HOOK3, RAB-interacting lysosomal protein (RILP), RAB11 family-interacting protein 3 (RAB11FIP3), ninein (NIN), and trafficking kinesin-binding protein 1 (TRAK1). Helix 1 is sufficient to bind RILP, whereas other adaptors require additional segments preceding helix 1 for efficient binding. Point mutations in the C-terminal helix 1 of Caenorhabditis elegans LIC, introduced by genome editing, severely affect development, locomotion, and life span of the animal and disrupt the distribution and transport kinetics of membrane cargo in axons of mechanosensory neurons, identical to what is observed when the entire LIC C-terminal region is deleted. Deletion of the C-terminal helix 2 delays dynein-dependent spindle positioning in the one-cell embryo but overall does not significantly perturb dynein function. We conclude that helix 1 in the intrinsically disordered region of LIC provides a conserved link between dynein and structurally diverse cargo adaptor families that is critical for dynein function in vivo.A highly conserved mechanism links the microtubule minus end–directed motor dynein to structurally diverse cargo adaptors through its light intermediate chain; this interaction is crucial for dynein function in vivo.Author summary: The large size and complex organization of animal cells make the correct and efficient distribution of intracellular content a challenge. The solution is to use motor proteins, which harness energy from ATP hydrolysis to walk along actin filaments or microtubules, for directional transport of cargo. The multi-subunit motor cytoplasmic dynein 1 (dynein) is responsible for transport directed toward the minus ends of microtubules. An important question is how dynein is recruited to its diverse cargo, which includes organelles such as endosomes and mitochondria, proteins, and mRNA. In this study, we use nuclear magnetic resonance spectroscopy to show that the light intermediate chain (LIC) subunit of human dynein uses a short helix in its disordered C-terminal region to bind structurally distinct adaptor proteins that connect the motor to specific cargo. We then use genome editing in the animal model C. elegans to demonstrate the functional relevance of the C-terminal LIC helix for dynein-dependent cargo transport in neurons. Thus, dynein recruitment to cargo involves a highly conserved interaction between LIC and adaptor proteins.

Suggested Citation

  • Ricardo Celestino & Morkos A Henen & José B Gama & Cátia Carvalho & Maxwell McCabe & Daniel J Barbosa & Alexandra Born & Parker J Nichols & Ana X Carvalho & Reto Gassmann & Beat Vögeli, 2019. "A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport," PLOS Biology, Public Library of Science, vol. 17(1), pages 1-33, January.
  • Handle: RePEc:plo:pbio00:3000100
    DOI: 10.1371/journal.pbio.3000100
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    References listed on IDEAS

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    1. In-Gyun Lee & Mara A. Olenick & Malgorzata Boczkowska & Clara Franzini-Armstrong & Erika L. F. Holzbaur & Roberto Dominguez, 2018. "A conserved interaction of the dynein light intermediate chain with dynein-dynactin effectors necessary for processivity," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    2. Rebecca B. Berlow & H. Jane Dyson & Peter E. Wright, 2017. "Hypersensitive termination of the hypoxic response by a disordered protein switch," Nature, Nature, vol. 543(7645), pages 447-451, March.
    3. Sagar P Mahale & Amit Sharma & Sivaram V S Mylavarapu, 2016. "Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint," PLOS ONE, Public Library of Science, vol. 11(7), pages 1-18, July.
    4. Alessandro Borgia & Madeleine B. Borgia & Katrine Bugge & Vera M. Kissling & Pétur O. Heidarsson & Catarina B. Fernandes & Andrea Sottini & Andrea Soranno & Karin J. Buholzer & Daniel Nettels & Birthe, 2018. "Extreme disorder in an ultrahigh-affinity protein complex," Nature, Nature, vol. 555(7694), pages 61-66, March.
    5. Linas Urnavicius & Clinton K. Lau & Mohamed M. Elshenawy & Edgar Morales-Rios & Carina Motz & Ahmet Yildiz & Andrew P. Carter, 2018. "Cryo-EM shows how dynactin recruits two dyneins for faster movement," Nature, Nature, vol. 554(7691), pages 202-206, February.
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