IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v554y2018i7691d10.1038_nature25462.html
   My bibliography  Save this article

Cryo-EM shows how dynactin recruits two dyneins for faster movement

Author

Listed:
  • Linas Urnavicius

    (Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue)

  • Clinton K. Lau

    (Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue)

  • Mohamed M. Elshenawy

    (University of California at Berkeley)

  • Edgar Morales-Rios

    (CINVESTAV)

  • Carina Motz

    (Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue
    Technische Universität München (TUM))

  • Ahmet Yildiz

    (University of California at Berkeley
    University of California at Berkeley)

  • Andrew P. Carter

    (Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue)

Abstract

Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. Here we use electron microscopy and single-molecule studies to show that adaptors can recruit a second dynein to dynactin. Whereas BICD2 is biased towards recruiting a single dynein, the adaptors BICDR1 and HOOK3 predominantly recruit two dyneins. We find that the shift towards a double dynein complex increases both the force and speed of the microtubule motor. Our 3.5 Å resolution cryo-electron microscopy reconstruction of a dynein tail–dynactin–BICDR1 complex reveals how dynactin can act as a scaffold to coordinate two dyneins side-by-side. Our work provides a structural basis for understanding how diverse adaptors recruit different numbers of dyneins and regulate the motile properties of the dynein–dynactin transport machine.

Suggested Citation

  • Linas Urnavicius & Clinton K. Lau & Mohamed M. Elshenawy & Edgar Morales-Rios & Carina Motz & Ahmet Yildiz & Andrew P. Carter, 2018. "Cryo-EM shows how dynactin recruits two dyneins for faster movement," Nature, Nature, vol. 554(7691), pages 202-206, February.
    • Handle: RePEc:nat:nature:v:554:y:2018:i:7691:d:10.1038_nature25462
    DOI: 10.1038/nature25462
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature25462
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature25462?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ricardo Celestino & Morkos A Henen & José B Gama & Cátia Carvalho & Maxwell McCabe & Daniel J Barbosa & Alexandra Born & Parker J Nichols & Ana X Carvalho & Reto Gassmann & Beat Vögeli, 2019. "A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport," PLOS Biology, Public Library of Science, vol. 17(1), pages 1-33, January.
    2. Kyoko Okada & Bharat R. Iyer & Lindsay G. Lammers & Pedro A. Gutierrez & Wenzhe Li & Steven M. Markus & Richard J. McKenney, 2023. "Conserved roles for the dynein intermediate chain and Ndel1 in assembly and activation of dynein," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    3. John T. Canty & Andrew Hensley & Merve Aslan & Amanda Jack & Ahmet Yildiz, 2023. "TRAK adaptors regulate the recruitment and activation of dynein and kinesin in mitochondrial transport," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:554:y:2018:i:7691:d:10.1038_nature25462. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.