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Conformational transitions of a neurotensin receptor 1–Gi1 complex

Author

Listed:
  • Hideaki E. Kato

    (Stanford University School of Medicine
    The University of Tokyo)

  • Yan Zhang

    (Stanford University School of Medicine
    Stanford University School of Medicine
    Zhejiang University School of Medicine)

  • Hongli Hu

    (Stanford University School of Medicine
    Stanford University School of Medicine)

  • Carl-Mikael Suomivuori

    (Stanford University School of Medicine
    Stanford University School of Medicine
    Stanford University
    Stanford University)

  • Francois Marie Ngako Kadji

    (Tohoku University)

  • Junken Aoki

    (Tohoku University)

  • Kaavya Krishna Kumar

    (Stanford University School of Medicine)

  • Rasmus Fonseca

    (Stanford University School of Medicine
    Stanford University School of Medicine
    University of Copenhagen)

  • Daniel Hilger

    (Stanford University School of Medicine)

  • Weijiao Huang

    (Stanford University School of Medicine)

  • Naomi R. Latorraca

    (Stanford University School of Medicine
    Stanford University School of Medicine
    Stanford University
    Stanford University)

  • Asuka Inoue

    (Tohoku University)

  • Ron O. Dror

    (Stanford University School of Medicine
    Stanford University School of Medicine
    Stanford University
    Stanford University)

  • Brian K. Kobilka

    (Stanford University School of Medicine)

  • Georgios Skiniotis

    (Stanford University School of Medicine
    Stanford University School of Medicine)

Abstract

Neurotensin receptor 1 (NTSR1) is a G-protein-coupled receptor (GPCR) that engages multiple subtypes of G protein, and is involved in the regulation of blood pressure, body temperature, weight and the response to pain. Here we present structures of human NTSR1 in complex with the agonist JMV449 and the heterotrimeric Gi1 protein, at a resolution of 3 Å. We identify two conformations: a canonical-state complex that is similar to recently reported GPCR–Gi/o complexes (in which the nucleotide-binding pocket adopts more flexible conformations that may facilitate nucleotide exchange), and a non-canonical state in which the G protein is rotated by about 45 degrees relative to the receptor and exhibits a more rigid nucleotide-binding pocket. In the non-canonical state, NTSR1 exhibits features of both active and inactive conformations, which suggests that the structure may represent an intermediate form along the activation pathway of G proteins. This structural information, complemented by molecular dynamics simulations and functional studies, provides insights into the complex process of G-protein activation.

Suggested Citation

  • Hideaki E. Kato & Yan Zhang & Hongli Hu & Carl-Mikael Suomivuori & Francois Marie Ngako Kadji & Junken Aoki & Kaavya Krishna Kumar & Rasmus Fonseca & Daniel Hilger & Weijiao Huang & Naomi R. Latorraca, 2019. "Conformational transitions of a neurotensin receptor 1–Gi1 complex," Nature, Nature, vol. 572(7767), pages 80-85, August.
  • Handle: RePEc:nat:nature:v:572:y:2019:i:7767:d:10.1038_s41586-019-1337-6
    DOI: 10.1038/s41586-019-1337-6
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