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Synchronized mitochondrial and cytosolic translation programs

Author

Listed:
  • Mary T. Couvillion

    (Harvard Medical School)

  • Iliana C. Soto

    (Harvard Medical School)

  • Gergana Shipkovenska

    (Harvard Medical School)

  • L. Stirling Churchman

    (Harvard Medical School)

Abstract

Oxidative phosphorylation (OXPHOS) is a vital process for energy generation, and is carried out by complexes within the mitochondria. OXPHOS complexes pose a unique challenge for cells because their subunits are encoded on both the nuclear and the mitochondrial genomes. Genomic approaches designed to study nuclear/cytosolic and bacterial gene expression have not been broadly applied to mitochondria, so the co-regulation of OXPHOS genes remains largely unexplored. Here we monitor mitochondrial and nuclear gene expression in Saccharomyces cerevisiae during mitochondrial biogenesis, when OXPHOS complexes are synthesized. We show that nuclear- and mitochondrial-encoded OXPHOS transcript levels do not increase concordantly. Instead, mitochondrial and cytosolic translation are rapidly, dynamically and synchronously regulated. Furthermore, cytosolic translation processes control mitochondrial translation unidirectionally. Thus, the nuclear genome coordinates mitochondrial and cytosolic translation to orchestrate the timely synthesis of OXPHOS complexes, representing an unappreciated regulatory layer shaping the mitochondrial proteome. Our whole-cell genomic profiling approach establishes a foundation for studies of global gene regulation in mitochondria.

Suggested Citation

  • Mary T. Couvillion & Iliana C. Soto & Gergana Shipkovenska & L. Stirling Churchman, 2016. "Synchronized mitochondrial and cytosolic translation programs," Nature, Nature, vol. 533(7604), pages 499-503, May.
  • Handle: RePEc:nat:nature:v:533:y:2016:i:7604:d:10.1038_nature18015
    DOI: 10.1038/nature18015
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    Cited by:

    1. Tang Cam Phung Pham & Steffen Henning Raun & Essi Havula & Carlos Henriquez-Olguín & Diana Rubalcava-Gracia & Emma Frank & Andreas Mæchel Fritzen & Paulo R. Jannig & Nicoline Resen Andersen & Rikke Kr, 2024. "The mitochondrial mRNA-stabilizing protein SLIRP regulates skeletal muscle mitochondrial structure and respiration by exercise-recoverable mechanisms," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    2. Eun-Ji Park & Hyun-Soo Kim & Do-Hyoung Lee & Su-Min Kim & Joon-Sup Yoon & Ji-Min Lee & Se Jin Im & Ho Lee & Min-Woo Lee & Chang-Woo Lee, 2023. "Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Abdul Haseeb Khan & Xuefang Gu & Rutvik J. Patel & Prabha Chuphal & Matheus P. Viana & Aidan I. Brown & Brian M. Zid & Tatsuhisa Tsuboi, 2024. "Mitochondrial protein heterogeneity stems from the stochastic nature of co-translational protein targeting in cell senescence," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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