IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v523y2015i7562d10.1038_nature14656.html
   My bibliography  Save this article

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Author

Listed:
  • Yanyong Kang

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • X. Edward Zhou

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Xiang Gao

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Yuanzheng He

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Wei Liu

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Andrii Ishchenko

    (Bridge Institute, University of Southern California)

  • Anton Barty

    (Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Thomas A. White

    (Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Oleksandr Yefanov

    (Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Gye Won Han

    (Bridge Institute, University of Southern California)

  • Qingping Xu

    (Joint Center for Structural Genomics, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory)

  • Parker W. de Waal

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Jiyuan Ke

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • M. H. Eileen Tan

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute
    Yong Loo Lin School of Medicine, National University of Singapore)

  • Chenghai Zhang

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Arne Moeller

    (The National Resource for Automated Molecular Microscopy, New York Structural Biology Center)

  • Graham M. West

    (The Scripps Research Institute, Scripps Florida)

  • Bruce D. Pascal

    (The Scripps Research Institute, Scripps Florida)

  • Ned Van Eps

    (University of California
    †Present address: Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.)

  • Lydia N. Caro

    (University of Toronto)

  • Sergey A. Vishnivetskiy

    (Vanderbilt University)

  • Regina J. Lee

    (Vanderbilt University)

  • Kelly M. Suino-Powell

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Xin Gu

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Kuntal Pal

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Jinming Ma

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Xiaoyong Zhi

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • Sébastien Boutet

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Garth J. Williams

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Marc Messerschmidt

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory
    BioXFEL, NSF Science and Technology Center)

  • Cornelius Gati

    (Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Nadia A. Zatsepin

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Arizona State University)

  • Dingjie Wang

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Arizona State University)

  • Daniel James

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Arizona State University)

  • Shibom Basu

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Arizona State University)

  • Shatabdi Roy-Chowdhury

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Arizona State University)

  • Chelsie E. Conrad

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Jesse Coe

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Haiguang Liu

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Beijing Computational Science Research Center, Haidian District)

  • Stella Lisova

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Christopher Kupitz

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    University of Wisconsin-Milwaukee)

  • Ingo Grotjohann

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Raimund Fromme

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Yi Jiang

    (State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences)

  • Minjia Tan

    (State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences)

  • Huaiyu Yang

    (State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences)

  • Jun Li

    (Yong Loo Lin School of Medicine, National University of Singapore)

  • Meitian Wang

    (Swiss Light Source at Paul Scherrer Institute)

  • Zhong Zheng

    (Bridge Institute, University of Southern California)

  • Dianfan Li

    (School of Medicine and School of Biochemistry and Immunology, Trinity College)

  • Nicole Howe

    (School of Medicine and School of Biochemistry and Immunology, Trinity College)

  • Yingming Zhao

    (BioXFEL, NSF Science and Technology Center
    University of Chicago)

  • Jörg Standfuss

    (Laboratory of Biomolecular Research at Paul Scherrer Institute)

  • Kay Diederichs

    (Universität Konstanz)

  • Yuhui Dong

    (Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences)

  • Clinton S. Potter

    (The National Resource for Automated Molecular Microscopy, New York Structural Biology Center)

  • Bridget Carragher

    (The National Resource for Automated Molecular Microscopy, New York Structural Biology Center)

  • Martin Caffrey

    (School of Medicine and School of Biochemistry and Immunology, Trinity College)

  • Hualiang Jiang

    (State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences)

  • Henry N. Chapman

    (Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
    Centre for Ultrafast Imaging)

  • John C. H. Spence

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Arizona State University)

  • Petra Fromme

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Uwe Weierstall

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University
    Arizona State University)

  • Oliver P. Ernst

    (University of Toronto
    University of Toronto)

  • Vsevolod Katritch

    (Bridge Institute, University of Southern California)

  • Vsevolod V. Gurevich

    (Vanderbilt University)

  • Patrick R. Griffin

    (The Scripps Research Institute, Scripps Florida)

  • Wayne L. Hubbell

    (University of California)

  • Raymond C. Stevens

    (Bridge Institute, University of Southern California
    Bridge Institute, University of Southern California
    iHuman Institute, ShanghaiTech University)

  • Vadim Cherezov

    (Bridge Institute, University of Southern California)

  • Karsten Melcher

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute)

  • H. Eric Xu

    (Laboratory of Structural Sciences, Center for Structural Biology and Drug Discovery, Van Andel Research Institute
    VARI-SIMM Center, Center for Structure and Function of Drug Targets, CAS-Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences)

Abstract

G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin–arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ∼20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology.

Suggested Citation

  • Yanyong Kang & X. Edward Zhou & Xiang Gao & Yuanzheng He & Wei Liu & Andrii Ishchenko & Anton Barty & Thomas A. White & Oleksandr Yefanov & Gye Won Han & Qingping Xu & Parker W. de Waal & Jiyuan Ke & , 2015. "Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser," Nature, Nature, vol. 523(7562), pages 561-567, July.
  • Handle: RePEc:nat:nature:v:523:y:2015:i:7562:d:10.1038_nature14656
    DOI: 10.1038/nature14656
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature14656
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.

    File URL: https://libkey.io/10.1038/nature14656?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Fenghui Zhao & Qingtong Zhou & Zhaotong Cong & Kaini Hang & Xinyu Zou & Chao Zhang & Yan Chen & Antao Dai & Anyi Liang & Qianqian Ming & Mu Wang & Li-Nan Chen & Peiyu Xu & Rulve Chang & Wenbo Feng & T, 2022. "Structural insights into multiplexed pharmacological actions of tirzepatide and peptide 20 at the GIP, GLP-1 or glucagon receptors," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Raphael S. Haider & Edda S. F. Matthees & Julia Drube & Mona Reichel & Ulrike Zabel & Asuka Inoue & Andy Chevigné & Cornelius Krasel & Xavier Deupi & Carsten Hoffmann, 2022. "β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    3. Kevin M. Dalton & Jack B. Greisman & Doeke R. Hekstra, 2022. "A unifying Bayesian framework for merging X-ray diffraction data," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Yasmin Aydin & Thore Böttke & Jordy Homing Lam & Stefan Ernicke & Anna Fortmann & Maik Tretbar & Barbara Zarzycka & Vsevolod V. Gurevich & Vsevolod Katritch & Irene Coin, 2023. "Structural details of a Class B GPCR-arrestin complex revealed by genetically encoded crosslinkers in living cells," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    5. Yutaro Shiraishi & Yutaka Kofuku & Takumi Ueda & Shubhi Pandey & Hemlata Dwivedi-Agnihotri & Arun K. Shukla & Ichio Shimada, 2021. "Biphasic activation of β-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR," Nature Communications, Nature, vol. 12(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:523:y:2015:i:7562:d:10.1038_nature14656. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.