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Conformational biosensors reveal GPCR signalling from endosomes

Author

Listed:
  • Roshanak Irannejad

    (University of California)

  • Jin C. Tomshine

    (University of California)

  • Jon R. Tomshine

    (University of California)

  • Michael Chevalier

    (University of California)

  • Jacob P. Mahoney

    (University of Michigan Medical School)

  • Jan Steyaert

    (Vrije Universiteit Brussel, B-1050 Brussels, Belgium
    Structural Biology Research Centre, VIB, B-1050 Brussels, Belgium)

  • Søren G. F. Rasmussen

    (The Panum Institute, University of Copenhagen, 2200 Copenhagen N, Denmark)

  • Roger K. Sunahara

    (University of Michigan Medical School)

  • Hana El-Samad

    (University of California)

  • Bo Huang

    (University of California
    University of California)

  • Mark von Zastrow

    (University of California
    University of California)

Abstract

Conformation-specific antibodies capable of monitoring the activation state of a G-protein-coupled seven-transmembrane receptor, the β2-adrenoceptor, reveals receptor and G-protein activation not only in the plasma membrane, but also in the endosome.

Suggested Citation

  • Roshanak Irannejad & Jin C. Tomshine & Jon R. Tomshine & Michael Chevalier & Jacob P. Mahoney & Jan Steyaert & Søren G. F. Rasmussen & Roger K. Sunahara & Hana El-Samad & Bo Huang & Mark von Zastrow, 2013. "Conformational biosensors reveal GPCR signalling from endosomes," Nature, Nature, vol. 495(7442), pages 534-538, March.
    • Handle: RePEc:nat:nature:v:495:y:2013:i:7442:d:10.1038_nature12000
    DOI: 10.1038/nature12000
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    Cited by:

    1. Shane C. Wright & Aikaterini Motso & Stefania Koutsilieri & Christian M. Beusch & Pierre Sabatier & Alessandro Berghella & Élodie Blondel-Tepaz & Kimberley Mangenot & Ioannis Pittarokoilis & Despoina-, 2023. "GLP-1R signaling neighborhoods associate with the susceptibility to adverse drug reactions of incretin mimetics," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Alexei Sirbu & Marc Bathe-Peters & Jothi L. M. Kumar & Asuka Inoue & Martin J. Lohse & Paolo Annibale, 2024. "Cell swelling enhances ligand-driven β-adrenergic signaling," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Baptiste Fischer & Tomasz Uchański & Aidana Sheryazdanova & Simon Gonzalez & Alexander N. Volkov & Elke Brosens & Thomas Zögg & Valentina Kalichuk & Steven Ballet & Wim Versées & Anna A. Sablina & Els, 2024. "Allosteric nanobodies to study the interactions between SOS1 and RAS," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    4. Dylan Scott Eiger & Noelia Boldizsar & Christopher Cole Honeycutt & Julia Gardner & Stephen Kirchner & Chloe Hicks & Issac Choi & Uyen Pham & Kevin Zheng & Anmol Warman & Jeffrey S. Smith & Jennifer Y, 2022. "Location bias contributes to functionally selective responses of biased CXCR3 agonists," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    5. Léa Ripoll & Yong Li & Carmen W. Dessauer & Mark Zastrow, 2024. "Spatial organization of adenylyl cyclase and its impact on dopamine signaling in neurons," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    6. Jun Yu & Amit Kumar & Xuefeng Zhang & Charlotte Martin & Kevin Van holsbeeck & Pierre Raia & Antoine Koehl & Toon Laeremans & Jan Steyaert & Aashish Manglik & Steven Ballet & Andreas Boland & Miriam S, 2024. "Structural basis of μ-opioid receptor targeting by a nanobody antagonist," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    7. Harrison M. York & Kunaal Joshi & Charles S. Wright & Laura Z. Kreplin & Samuel J. Rodgers & Ullhas K. Moorthi & Hetvi Gandhi & Abhishek Patil & Christina A. Mitchell & Srividya Iyer-Biswas & Senthil , 2023. "Deterministic early endosomal maturations emerge from a stochastic trigger-and-convert mechanism," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    8. Mithu Baidya & Madhu Chaturvedi & Hemlata Dwivedi-Agnihotri & Ashutosh Ranjan & Dominic Devost & Yoon Namkung & Tomasz Maciej Stepniewski & Shubhi Pandey & Minakshi Baruah & Bhanupriya Panigrahi & Par, 2022. "Allosteric modulation of GPCR-induced β-arrestin trafficking and signaling by a synthetic intrabody," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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