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Endothelial and perivascular cells maintain haematopoietic stem cells

Author

Listed:
  • Lei Ding

    (Howard Hughes Medical Institute, Children’s Research Institute, University of Texas Southwestern Medical Center)

  • Thomas L. Saunders

    (Transgenic Animal Model Core, University of Michigan)

  • Grigori Enikolopov

    (Cold Spring Harbor Laboratory)

  • Sean J. Morrison

    (Howard Hughes Medical Institute, Children’s Research Institute, University of Texas Southwestern Medical Center)

Abstract

Several cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem cell factor (SCF; also known as KITL) is a key niche component that maintains HSCs. Here, using Scfgfp knock-in mice, we found that Scf was primarily expressed by perivascular cells throughout the bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, nestin-cre- or nestin-creER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. Thus, HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance.

Suggested Citation

  • Lei Ding & Thomas L. Saunders & Grigori Enikolopov & Sean J. Morrison, 2012. "Endothelial and perivascular cells maintain haematopoietic stem cells," Nature, Nature, vol. 481(7382), pages 457-462, January.
    • Handle: RePEc:nat:nature:v:481:y:2012:i:7382:d:10.1038_nature10783
    DOI: 10.1038/nature10783
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