IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v460y2009i7259d10.1038_nature08285.html
   My bibliography  Save this article

Immortalization eliminates a roadblock during cellular reprogramming into iPS cells

Author

Listed:
  • Jochen Utikal

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA
    Venereology and Allergology, University Medical Center Mannheim, Ruprecht-Karl-University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68135 Mannheim, Germany)

  • Jose M. Polo

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA)

  • Matthias Stadtfeld

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA)

  • Nimet Maherali

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA
    Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA)

  • Warakorn Kulalert

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA)

  • Ryan M. Walsh

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA)

  • Adam Khalil

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA)

  • James G. Rheinwald

    (Brigham and Women’s Hospital and Harvard Skin Disease Research Center, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA)

  • Konrad Hochedlinger

    (Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Harvard University, Cambridge, Massachusetts 02138, USA)

Abstract

On iPS cells and p53: removing the roadblock Pluripotency can be induced in somatic cells by overexpression of a set of transcription factors. The process has extremely low efficiency and slow kinetics. Here Utikal et al. show that cells with low endogenous p19Arf levels and immortal fibroblasts deficient for components of the Ink4a/Arf/p53 pathway yield iPS colonies with a threefold faster kinetics and at a significantly higher efficiency compared with wild-type cells, reaching frequencies of up to 100%. Genetic deletion of p53 in cellular subpopulations that normally fail to reprogram rescues their ability to produce iPS cells.

Suggested Citation

  • Jochen Utikal & Jose M. Polo & Matthias Stadtfeld & Nimet Maherali & Warakorn Kulalert & Ryan M. Walsh & Adam Khalil & James G. Rheinwald & Konrad Hochedlinger, 2009. "Immortalization eliminates a roadblock during cellular reprogramming into iPS cells," Nature, Nature, vol. 460(7259), pages 1145-1148, August.
    • Handle: RePEc:nat:nature:v:460:y:2009:i:7259:d:10.1038_nature08285
    DOI: 10.1038/nature08285
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature08285
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature08285?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Tomoe Ueyama & Shu Nakao & Tasuku Tsukamoto & Dai Ihara & Yukihiro Harada & Yuka Akagi & Sae Nakagawa & Teruhisa Kawamura & Takahiro Sogo & Yasuyuki S Kida, 2018. "PTEN/Akt Axis is Involved in Somatic Cell Reprogramming to Mouse iPS Cells," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 11(5), pages 8789-8795, December.
    2. Siti Razila Abdul Razak & Kazuko Ueno & Naoya Takayama & Naoki Nariai & Masao Nagasaki & Rika Saito & Hideto Koso & Chen-Yi Lai & Miyako Murakami & Koichiro Tsuji & Tatsuo Michiue & Hiromitsu Nakauchi, 2013. "Profiling of MicroRNA in Human and Mouse ES and iPS Cells Reveals Overlapping but Distinct MicroRNA Expression Patterns," PLOS ONE, Public Library of Science, vol. 8(9), pages 1-16, September.
    3. Patricia Gerdes & Sue Mei Lim & Adam D. Ewing & Michael R. Larcombe & Dorothy Chan & Francisco J. Sanchez-Luque & Lucinda Walker & Alexander L. Carleton & Cini James & Anja S. Knaupp & Patricia E. Car, 2022. "Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:460:y:2009:i:7259:d:10.1038_nature08285. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.