IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v460y2009i7251d10.1038_nature08152.html
   My bibliography  Save this article

Cells keep a memory of their tissue origin during axolotl limb regeneration

Author

Listed:
  • Martin Kragl

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108,
    Center for Regenerative Therapies, University of Technology Dresden, Tatzberg 47/49, 01307 Dresden, Germany
    Present address: Institute of Metabolic Physiology, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, 40225 Düsseldorf, Germany.)

  • Dunja Knapp

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108,
    Center for Regenerative Therapies, University of Technology Dresden, Tatzberg 47/49, 01307 Dresden, Germany)

  • Eugen Nacu

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108,
    Center for Regenerative Therapies, University of Technology Dresden, Tatzberg 47/49, 01307 Dresden, Germany)

  • Shahryar Khattak

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108,
    Center for Regenerative Therapies, University of Technology Dresden, Tatzberg 47/49, 01307 Dresden, Germany)

  • Malcolm Maden

    (The Regeneration Project, McKnight Brian Institute, University of Florida, Rm 326 Bartram Hall, Gainesville, Florida 32611, USA)

  • Hans Henning Epperlein

    (Institute of Anatomy, Medical Faculty, University of Technology Dresden, Fetscherstrasse 74,)

  • Elly M. Tanaka

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108,
    Center for Regenerative Therapies, University of Technology Dresden, Tatzberg 47/49, 01307 Dresden, Germany)

Abstract

During limb regeneration adult tissue is converted into a zone of undifferentiated progenitors called the blastema that reforms the diverse tissues of the limb. Previous experiments have led to wide acceptance that limb tissues dedifferentiate to form pluripotent cells. Here we have reexamined this question using an integrated GFP transgene to track the major limb tissues during limb regeneration in the salamander Ambystoma mexicanum (the axolotl). Surprisingly, we find that each tissue produces progenitor cells with restricted potential. Therefore, the blastema is a heterogeneous collection of restricted progenitor cells. On the basis of these findings, we further demonstrate that positional identity is a cell-type-specific property of blastema cells, in which cartilage-derived blastema cells harbour positional identity but Schwann-derived cells do not. Our results show that the complex phenomenon of limb regeneration can be achieved without complete dedifferentiation to a pluripotent state, a conclusion with important implications for regenerative medicine.

Suggested Citation

  • Martin Kragl & Dunja Knapp & Eugen Nacu & Shahryar Khattak & Malcolm Maden & Hans Henning Epperlein & Elly M. Tanaka, 2009. "Cells keep a memory of their tissue origin during axolotl limb regeneration," Nature, Nature, vol. 460(7251), pages 60-65, July.
    • Handle: RePEc:nat:nature:v:460:y:2009:i:7251:d:10.1038_nature08152
    DOI: 10.1038/nature08152
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature08152
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature08152?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Béryl Laplace-Builhé & Audrey Barthelaix & Said Assou & Candice Bohaud & Marine Pratlong & Dany Severac & Gautier Tejedor & Patricia Luz-Crawford & Mai Nguyen-Chi & Marc Mathieu & Christian Jorgensen , 2021. "NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Thomas P. Lozito & Ricardo Londono & Aaron X. Sun & Megan L. Hudnall, 2021. "Introducing dorsoventral patterning in adult regenerating lizard tails with gene-edited embryonic neural stem cells," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    3. Jixing Zhong & Rita Aires & Georgios Tsissios & Evangelia Skoufa & Kerstin Brandt & Tatiana Sandoval-Guzmán & Can Aztekin, 2023. "Multi-species atlas resolves an axolotl limb development and regeneration paradox," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    4. Daniel H Rapoport & Tim Becker & Amir Madany Mamlouk & Simone Schicktanz & Charli Kruse, 2011. "A Novel Validation Algorithm Allows for Automated Cell Tracking and the Extraction of Biologically Meaningful Parameters," PLOS ONE, Public Library of Science, vol. 6(11), pages 1-16, November.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:460:y:2009:i:7251:d:10.1038_nature08152. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.