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NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration

Author

Listed:
  • Béryl Laplace-Builhé

    (IRMB, Univ Montpellier, INSERM)

  • Audrey Barthelaix

    (IRMB, Univ Montpellier, INSERM)

  • Said Assou

    (IRMB, Univ Montpellier, INSERM)

  • Candice Bohaud

    (IRMB, Univ Montpellier, INSERM)

  • Marine Pratlong

    (MGX, BCM, Univ Montpellier, CNRS, INSERM)

  • Dany Severac

    (MGX, BCM, Univ Montpellier, CNRS, INSERM)

  • Gautier Tejedor

    (IRMB, Univ Montpellier, INSERM)

  • Patricia Luz-Crawford

    (Universidad de los Andes
    IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy)

  • Mai Nguyen-Chi

    (LPHI, Univ Montpellier, CNRS)

  • Marc Mathieu

    (IRMB, Univ Montpellier, INSERM)

  • Christian Jorgensen

    (IRMB, Univ Montpellier, INSERM
    CHU Montpellier)

  • Farida Djouad

    (IRMB, Univ Montpellier, INSERM)

Abstract

Fish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.

Suggested Citation

  • Béryl Laplace-Builhé & Audrey Barthelaix & Said Assou & Candice Bohaud & Marine Pratlong & Dany Severac & Gautier Tejedor & Patricia Luz-Crawford & Mai Nguyen-Chi & Marc Mathieu & Christian Jorgensen , 2021. "NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26422-5
    DOI: 10.1038/s41467-021-26422-5
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    References listed on IDEAS

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    1. Nicholas D. Leigh & Garrett S. Dunlap & Kimberly Johnson & Rachelle Mariano & Rachel Oshiro & Alan Y. Wong & Donald M. Bryant & Bess M. Miller & Alex Ratner & Andy Chen & William W. Ye & Brian J. Haas, 2018. "Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    2. Martin Kragl & Dunja Knapp & Eugen Nacu & Shahryar Khattak & Malcolm Maden & Hans Henning Epperlein & Elly M. Tanaka, 2009. "Cells keep a memory of their tissue origin during axolotl limb regeneration," Nature, Nature, vol. 460(7251), pages 60-65, July.
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