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High-resolution mapping of meiotic crossovers and non-crossovers in yeast

Author

Listed:
  • Eugenio Mancera

    (European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany)

  • Richard Bourgon

    (European Molecular Biology Laboratory, European Bioinformatics Institute)

  • Alessandro Brozzi

    (European Molecular Biology Laboratory, European Bioinformatics Institute)

  • Wolfgang Huber

    (European Molecular Biology Laboratory, European Bioinformatics Institute)

  • Lars M. Steinmetz

    (European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany)

Abstract

Meiotic recombination has a central role in the evolution of sexually reproducing organisms. The two recombination outcomes, crossover and non-crossover, increase genetic diversity, but have the potential to homogenize alleles by gene conversion. Whereas crossover rates vary considerably across the genome, non-crossovers and gene conversions have only been identified in a handful of loci. To examine recombination genome wide and at high spatial resolution, we generated maps of crossovers, crossover-associated gene conversion and non-crossover gene conversion using dense genetic marker data collected from all four products of fifty-six yeast (Saccharomyces cerevisiae) meioses. Our maps reveal differences in the distributions of crossovers and non-crossovers, showing more regions where either crossovers or non-crossovers are favoured than expected by chance. Furthermore, we detect evidence for interference between crossovers and non-crossovers, a phenomenon previously only known to occur between crossovers. Up to 1% of the genome of each meiotic product is subject to gene conversion in a single meiosis, with detectable bias towards GC nucleotides. To our knowledge the maps represent the first high-resolution, genome-wide characterization of the multiple outcomes of recombination in any organism. In addition, because non-crossover hotspots create holes of reduced linkage within haplotype blocks, our results stress the need to incorporate non-crossovers into genetic linkage analysis.

Suggested Citation

  • Eugenio Mancera & Richard Bourgon & Alessandro Brozzi & Wolfgang Huber & Lars M. Steinmetz, 2008. "High-resolution mapping of meiotic crossovers and non-crossovers in yeast," Nature, Nature, vol. 454(7203), pages 479-485, July.
    • Handle: RePEc:nat:nature:v:454:y:2008:i:7203:d:10.1038_nature07135
    DOI: 10.1038/nature07135
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    Cited by:

    1. Stuart D. Desjardins & James Simmonds & Inna Guterman & Kostya Kanyuka & Amanda J. Burridge & Andrew J. Tock & Eugenio Sanchez-Moran & F. Chris H. Franklin & Ian R. Henderson & Keith J. Edwards & Cris, 2022. "FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Taikui Zhang & Weichen Huang & Lin Zhang & De-Zhu Li & Ji Qi & Hong Ma, 2024. "Phylogenomic profiles of whole-genome duplications in Poaceae and landscape of differential duplicate retention and losses among major Poaceae lineages," Nature Communications, Nature, vol. 15(1), pages 1-27, December.
    3. Simone Mozzachiodi & Lorenzo Tattini & Agnes Llored & Agurtzane Irizar & Neža Škofljanc & Melania D’Angiolo & Matteo De Chiara & Benjamin P. Barré & Jia-Xing Yue & Angela Lutazi & Sophie Loeillet & Ra, 2021. "Aborting meiosis allows recombination in sterile diploid yeast hybrids," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    4. Qichao Lian & Victor Solier & Birgit Walkemeier & Stéphanie Durand & Bruno Huettel & Korbinian Schneeberger & Raphael Mercier, 2022. "The megabase-scale crossover landscape is largely independent of sequence divergence," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    5. Marcel Ernst & Raphael Mercier & David Zwicker, 2024. "Interference length reveals regularity of crossover placement across species," Nature Communications, Nature, vol. 15(1), pages 1-9, December.

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