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Reactive oxygen species produced by NADPH oxidase regulate plant cell growth

Author

Listed:
  • Julia Foreman

    (John Innes Centre)

  • Vadim Demidchik

    (University of Cambridge)

  • John H. F. Bothwell

    (Marine Biological Association)

  • Panagiota Mylona

    (John Innes Centre)

  • Henk Miedema

    (University of Cambridge)

  • Miguel Angel Torres

    (Sainsbury Laboratory, John Innes Centre
    University of North Carolina)

  • Paul Linstead

    (John Innes Centre)

  • Silvia Costa

    (John Innes Centre)

  • Colin Brownlee

    (Marine Biological Association)

  • Jonathan D. G. Jones

    (Sainsbury Laboratory, John Innes Centre)

  • Julia M. Davies

    (University of Cambridge)

  • Liam Dolan

    (John Innes Centre)

Abstract

Cell expansion is a central process in plant morphogenesis, and the elongation of roots and root hairs is essential for uptake of minerals and water from the soil. Ca2+ influx from the extracellular store is required for (and sets the rates of) cell elongation in roots1. Arabidopsis thaliana rhd2 mutants are defective in Ca2+ uptake and consequently cell expansion is compromised—rhd2 mutants have short root hairs2,3 and stunted roots. To determine the regulation of Ca2+ acquisition in growing root cells we show here that RHD2 is an NADPH oxidase, a protein that transfers electrons from NADPH to an electron acceptor leading to the formation of reactive oxygen species (ROS). We show that ROS accumulate in growing wild-type (WT) root hairs but their levels are markedly decreased in rhd2 mutants. Blocking the activity of the NADPH oxidase with diphenylene iodonium (DPI) inhibits ROS formation and phenocopies Rhd2-. Treatment of rhd2 roots with ROS partly suppresses the mutant phenotype and stimulates the activity of plasma membrane hyperpolarization-activated Ca2+ channels, the predominant root Ca2+ acquisition system. This indicates that NADPH oxidases control development by making ROS that regulate plant cell expansion through the activation of Ca2+ channels.

Suggested Citation

  • Julia Foreman & Vadim Demidchik & John H. F. Bothwell & Panagiota Mylona & Henk Miedema & Miguel Angel Torres & Paul Linstead & Silvia Costa & Colin Brownlee & Jonathan D. G. Jones & Julia M. Davies &, 2003. "Reactive oxygen species produced by NADPH oxidase regulate plant cell growth," Nature, Nature, vol. 422(6930), pages 442-446, March.
  • Handle: RePEc:nat:nature:v:422:y:2003:i:6930:d:10.1038_nature01485
    DOI: 10.1038/nature01485
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    Cited by:

    1. Longjia Tian & Guangcheng Shao & Yang Gao & Enze Song & Jia Lu, 2024. "Effects of Biochar on Soil Organic Carbon in Relation to Soil Nutrient Contents, Climate Zones and Cropping Systems: A Chinese Meta-Analysis," Land, MDPI, vol. 13(10), pages 1-18, October.
    2. Liao, Qi & Ding, Risheng & Du, Taisheng & Kang, Shaozhong & Tong, Ling & Li, Sien, 2022. "Stomatal conductance drives variations of yield and water use of maize under water and nitrogen stress," Agricultural Water Management, Elsevier, vol. 268(C).
    3. Z.B. Qiu & Q. Li & Z.Z. Bi & M. Yue, 2011. "Hydrogen peroxide acts as a signal molecule in CO2 laser pretreatment-induced osmotic tolerance in wheat seedling," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 57(9), pages 403-408.
    4. Geng Tian & Shubin Wang & Jianhui Wu & Yanxia Wang & Xiutang Wang & Shuwei Liu & Dejun Han & Guangmin Xia & Mengcheng Wang, 2023. "Allelic variation of TaWD40-4B.1 contributes to drought tolerance by modulating catalase activity in wheat," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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