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GTPase activity of dynamin and resulting conformation change are essential for endocytosis

Author

Listed:
  • Bruno Marks

    (MRC Laboratory of Molecular Biology)

  • Michael H. B. Stowell

    (MRC Laboratory of Molecular Biology)

  • Yvonne Vallis

    (MRC Laboratory of Molecular Biology)

  • Ian G. Mills

    (MRC Laboratory of Molecular Biology)

  • Adele Gibson

    (MRC Laboratory for Molecular Cell Biology, University College London)

  • Colin R. Hopkins

    (MRC Laboratory for Molecular Cell Biology, University College London)

  • Harvey T. McMahon

    (MRC Laboratory of Molecular Biology)

Abstract

Dynamin is a large GTPase with a relative molecular mass of 96,000 (Mr 96K) that is involved in clathrin-mediated endocytosis and other vesicular trafficking processes1,2. Although its function is apparently essential for scission of newly formed vesicles from the plasma membrane, the nature of dynamin's role in the scission process is still unclear3,4. It has been proposed that dynamin is a regulator (similar to classical G proteins) of downstream effectors5. Here we report the analysis of several point mutants of dynamin's GTPase effector (GED) and GTPase domains. We show that oligomerization and GTP binding alone, by dynamin, are not sufficient for endocytosis in vivo. Rather, efficient GTP hydrolysis and an associated conformational change are also required. These data argue that dynamin has a mechanochemical function in vesicle scission.

Suggested Citation

  • Bruno Marks & Michael H. B. Stowell & Yvonne Vallis & Ian G. Mills & Adele Gibson & Colin R. Hopkins & Harvey T. McMahon, 2001. "GTPase activity of dynamin and resulting conformation change are essential for endocytosis," Nature, Nature, vol. 410(6825), pages 231-235, March.
    • Handle: RePEc:nat:nature:v:410:y:2001:i:6825:d:10.1038_35065645
    DOI: 10.1038/35065645
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    Cited by:

    1. Katherine Bonnycastle & Katharine L. Dobson & Eva-Maria Blumrich & Akshada Gajbhiye & Elizabeth C. Davenport & Marie Pronot & Moritz Steinruecke & Matthias Trost & Alfredo Gonzalez-Sulser & Michael A., 2023. "Reversal of cell, circuit and seizure phenotypes in a mouse model of DNM1 epileptic encephalopathy," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. David Paul & Omer Stern & Yvonne Vallis & Jatinder Dhillon & Andrew Buchanan & Harvey McMahon, 2023. "Cell surface protein aggregation triggers endocytosis to maintain plasma membrane proteostasis," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Anmin Jiang & Kye Kudo & Rachel S. Gormal & Sevannah Ellis & Sikao Guo & Tristan P. Wallis & Shanley F. Longfield & Phillip J. Robinson & Margaret E. Johnson & Merja Joensuu & Frédéric A. Meunier, 2024. "Dynamin1 long- and short-tail isoforms exploit distinct recruitment and spatial patterns to form endocytic nanoclusters," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

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