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A structural change in the kinesin motor protein that drives motility

Author

Listed:
  • Sarah Rice

    (Departments of Cellular and Molecular Pharmacology)

  • Abel W. Lin

    (The Scripps Research Institute)

  • Daniel Safer

    (University of Pennsylvania School of Medicine)

  • Cynthia L. Hart

    (The Howard Hughes Medical Institute)

  • Nariman Naber

    (Biochemistry and Biophysics, University of California)

  • Bridget O. Carragher

    (Beckman Institute, University of Illinois at Urbana-Champaign)

  • Shane M. Cain

    (The Scripps Research Institute)

  • Elena Pechatnikova

    (University of Chicago)

  • Elizabeth M. Wilson-Kubalek

    (The Scripps Research Institute)

  • Michael Whittaker

    (The Scripps Research Institute)

  • Edward Pate

    (Washington State University)

  • Roger Cooke

    (Biochemistry and Biophysics, University of California)

  • Edwin W. Taylor

    (University of Chicago)

  • Ronald A. Milligan

    (The Scripps Research Institute)

  • Ronald D. Vale

    (The Howard Hughes Medical Institute
    Departments of Cellular and Molecular Pharmacology)

Abstract

Kinesin motors power many motile processes by converting ATP energy into unidirectional motion along microtubules. The force-generating and enzymatic properties of conventional kinesin have been extensively studied; however, the structural basis of movement is unknown. Here we have detected and visualized a large conformational change of a ∼15-amino-acid region (the neck linker) in kinesin using electron paramagnetic resonance, fluorescence resonance energy transfer, pre-steady state kinetics and cryo-electron microscopy. This region becomes immobilized and extended towards the microtubule ‘plus’ end when kinesin binds microtubules and ATP, and reverts to a more mobile conformation when γ-phosphate is released after nucleotide hydrolysis. This conformational change explains both the direction of kinesin motion and processive movement by the kinesin dimer.

Suggested Citation

  • Sarah Rice & Abel W. Lin & Daniel Safer & Cynthia L. Hart & Nariman Naber & Bridget O. Carragher & Shane M. Cain & Elena Pechatnikova & Elizabeth M. Wilson-Kubalek & Michael Whittaker & Edward Pate & , 1999. "A structural change in the kinesin motor protein that drives motility," Nature, Nature, vol. 402(6763), pages 778-784, December.
    • Handle: RePEc:nat:nature:v:402:y:1999:i:6763:d:10.1038_45483
    DOI: 10.1038/45483
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    Citations

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    Cited by:

    1. Takema Sasaki & Kei Saito & Daisuke Inoue & Henrik Serk & Yuki Sugiyama & Edouard Pesquet & Yuta Shimamoto & Yoshihisa Oda, 2023. "Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Chunting Zhang & Changmiao Guo & Ryan W. Russell & Caitlin M. Quinn & Mingyue Li & John C. Williams & Angela M. Gronenborn & Tatyana Polenova, 2022. "Magic-angle-spinning NMR structure of the kinesin-1 motor domain assembled with microtubules reveals the elusive neck linker orientation," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Ju Zhou & Anhui Wang & Yinlong Song & Nan Liu & Jia Wang & Yan Li & Xin Liang & Guohui Li & Huiying Chu & Hong-Wei Wang, 2023. "Structural insights into the mechanism of GTP initiation of microtubule assembly," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    4. Mireia Andreu-Carbó & Cornelia Egoldt & Marie-Claire Velluz & Charlotte Aumeier, 2024. "Microtubule damage shapes the acetylation gradient," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    5. Matthieu P. M. H. Benoit & Lu Rao & Ana B. Asenjo & Arne Gennerich & Hernando Sosa, 2024. "Cryo-EM unveils kinesin KIF1A’s processivity mechanism and the impact of its pathogenic variant P305L," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    6. Doan Tuong-Van Le & Thomas Eckert & Günther Woehlke, 2013. "Computer Simulation of Assembly and Co-operativity of Hexameric AAA ATPases," PLOS ONE, Public Library of Science, vol. 8(7), pages 1-19, July.
    7. Guido Scarabelli & Barry J Grant, 2013. "Mapping the Structural and Dynamical Features of Kinesin Motor Domains," PLOS Computational Biology, Public Library of Science, vol. 9(11), pages 1-13, November.
    8. Tianyang Liu & Fiona Shilliday & Alexander D. Cook & Mohammad Zeeshan & Declan Brady & Rita Tewari & Colin J. Sutherland & Anthony J. Roberts & Carolyn A. Moores, 2022. "Mechanochemical tuning of a kinesin motor essential for malaria parasite transmission," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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