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Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain

Author

Listed:
  • Eric H. Schroeter

    (Washington University)

  • Jeffrey A. Kisslinger

    (Washington University)

  • Raphael Kopan

    (Washington University)

Abstract

Notch proteins are ligand-activated transmembrane receptors involved in cell-fate selection throughout development1,2,3. No known enzymatic activity is contained within Notch and the molecular mechanism by which it transduces signals across the cell membrane is poorly understood. In many instances, Notch activation results in transcriptional changes in the nucleus through an association with members of the CSL family of DNA-binding proteins (where CSL stands for CBF1, Su(H), Lag-1)1,2,3,4. As Notch is located in the plasma membrane and CSL is a nuclear protein, two models have been proposed to explain how they interact (Fig. 1) . The first suggests that the two interact transiently at the membrane1,5,6,7. The second postulates that Notch is cleaved by a protease, enabling the cleaved fragment to enter the nucleus6,8,9,10,11,12,13,14. Here we show that signalling by a constitutively active membrane-bound Notch-1 protein requires the proteolytic release of the Notch intracellular domain (NICD), which interacts preferentially with CSL. Very small amounts of NICD are active, explaining why it is hard to detect in the nucleus in vivo. We also show that it is ligand binding that induces release of NICD. Figure 1 Different predictions are made by two models of Notch signalling. a, Models not invoking processing propose that interactions between Notch and CSL at the membrane may be sufficient to transduce a signal. Thus, ligand-regulated NICD release is not expected to be necessary for signalling. b, The processing model suggests that Notch signalling requires the release of NICD, which is capable of direct interaction with CSL in the nucleus, to turn on transcription of target genes. This model predicts that NICD release is regulated by ligand binding and that blocking proteolysis interferes with signalling. Notch-1 domain symbols as in Fig. 2.

Suggested Citation

  • Eric H. Schroeter & Jeffrey A. Kisslinger & Raphael Kopan, 1998. "Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain," Nature, Nature, vol. 393(6683), pages 382-386, May.
  • Handle: RePEc:nat:nature:v:393:y:1998:i:6683:d:10.1038_30756
    DOI: 10.1038/30756
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    Cited by:

    1. Hongrui Wang & Liang Yu & Jin’e Wang & Yaqing Zhang & Mengchen Xu & Cheng Lv & Bing Cui & Mengmeng Yuan & Yu Zhang & Yupeng Yan & Rutai Hui & Yibo Wang, 2023. "SLC35D3 promotes white adipose tissue browning to ameliorate obesity by NOTCH signaling," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Ioanna Smyrlaki & Ferenc Fördős & Iris Rocamonde-Lago & Yang Wang & Boxuan Shen & Antonio Lentini & Vincent C. Luca & Björn Reinius & Ana I. Teixeira & Björn Högberg, 2024. "Soluble and multivalent Jag1 DNA origami nanopatterns activate Notch without pulling force," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Soraia Barão & Yijun Xu & José P. Llongueras & Rachel Vistein & Loyal Goff & Kristina J. Nielsen & Byoung-Il Bae & Richard S. Smith & Christopher A. Walsh & Genevieve Stein-O’Brien & Ulrich Müller, 2024. "Conserved transcriptional regulation by BRN1 and BRN2 in neocortical progenitors drives mammalian neural specification and neocortical expansion," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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