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The C-terminal domain of RNA polymerase II couples mRNA processing to transcription

Author

Listed:
  • Susan McCracken

    (Amgen Institute)

  • Nova Fong

    (Amgen Institute)

  • Krassimir Yankulov

    (Amgen Institute)

  • Scott Ballantyne

    (Amgen Institute)

  • Guohua Pan

    (Amgen Institute)

  • Jack Greenblatt

    (Amgen Institute)

  • Scott D. Patterson

    (Amgen Institute)

  • Marvin Wickens

    (Amgen Institute)

  • David L. Bentley

    (Amgen Institute)

Abstract

Messenger RNA is produced by RNA polymerase II (pol II) transcription, followed by processing of the primary transcript. Transcription, splicing and cleavage–polyadenylation can occur independently in vitro, but we demonstrate here that these processes are intimately linked in vivo. We show that the carboxy-terminal domain (CTD) of the pol II large subunit is required for efficient RNA processing. Splicing, processing of the 3′ end and termination of transcription downstream of the poly(A) site, are all inhibited by truncation of the CTD. We found that the cleavage–polyadenylation factors CPSF and CstF specifically bound to CTD affinity columns and copurified with pol II in a high-molecular-mass complex. Our demonstration of an association between the CTD and 3′-processing factors, considered together with reports of a similar interaction with splicing factors1,2, suggests that an mRNA 'factory' exists which carries out coupled transcription, splicing and cleavage–polyadenylation of mRNA precursors.

Suggested Citation

  • Susan McCracken & Nova Fong & Krassimir Yankulov & Scott Ballantyne & Guohua Pan & Jack Greenblatt & Scott D. Patterson & Marvin Wickens & David L. Bentley, 1997. "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription," Nature, Nature, vol. 385(6614), pages 357-361, January.
    • Handle: RePEc:nat:nature:v:385:y:1997:i:6614:d:10.1038_385357a0
    DOI: 10.1038/385357a0
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    Cited by:

    1. Katerina Linhartova & Francesco Luca Falginella & Martin Matl & Marek Sebesta & Robert Vácha & Richard Stefl, 2024. "Sequence and structural determinants of RNAPII CTD phase-separation and phosphorylation by CDK7," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Buki Kwon & Mervin M. Fansler & Neil D. Patel & Jihye Lee & Weirui Ma & Christine Mayr, 2022. "Enhancers regulate 3′ end processing activity to control expression of alternative 3′UTR isoforms," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Yanfen Zheng & Xingyang Li & Shuang Deng & Hongzhe Zhao & Ying Ye & Shaoping Zhang & Xudong Huang & Ruihong Bai & Lisha Zhuang & Quanbo Zhou & Mei Li & Jiachun Su & Rui Li & Xiaoqiong Bao & Lingxing Z, 2023. "CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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