IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v385y1997i6614d10.1038_385357a0.html
   My bibliography  Save this article

The C-terminal domain of RNA polymerase II couples mRNA processing to transcription

Author

Listed:
  • Susan McCracken

    (Amgen Institute)

  • Nova Fong

    (Amgen Institute)

  • Krassimir Yankulov

    (Amgen Institute)

  • Scott Ballantyne

    (Amgen Institute)

  • Guohua Pan

    (Amgen Institute)

  • Jack Greenblatt

    (Amgen Institute)

  • Scott D. Patterson

    (Amgen Institute)

  • Marvin Wickens

    (Amgen Institute)

  • David L. Bentley

    (Amgen Institute)

Abstract

Messenger RNA is produced by RNA polymerase II (pol II) transcription, followed by processing of the primary transcript. Transcription, splicing and cleavage–polyadenylation can occur independently in vitro, but we demonstrate here that these processes are intimately linked in vivo. We show that the carboxy-terminal domain (CTD) of the pol II large subunit is required for efficient RNA processing. Splicing, processing of the 3′ end and termination of transcription downstream of the poly(A) site, are all inhibited by truncation of the CTD. We found that the cleavage–polyadenylation factors CPSF and CstF specifically bound to CTD affinity columns and copurified with pol II in a high-molecular-mass complex. Our demonstration of an association between the CTD and 3′-processing factors, considered together with reports of a similar interaction with splicing factors1,2, suggests that an mRNA 'factory' exists which carries out coupled transcription, splicing and cleavage–polyadenylation of mRNA precursors.

Suggested Citation

  • Susan McCracken & Nova Fong & Krassimir Yankulov & Scott Ballantyne & Guohua Pan & Jack Greenblatt & Scott D. Patterson & Marvin Wickens & David L. Bentley, 1997. "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription," Nature, Nature, vol. 385(6614), pages 357-361, January.
    • Handle: RePEc:nat:nature:v:385:y:1997:i:6614:d:10.1038_385357a0
    DOI: 10.1038/385357a0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/385357a0
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/385357a0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Buki Kwon & Mervin M. Fansler & Neil D. Patel & Jihye Lee & Weirui Ma & Christine Mayr, 2022. "Enhancers regulate 3′ end processing activity to control expression of alternative 3′UTR isoforms," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Yanfen Zheng & Xingyang Li & Shuang Deng & Hongzhe Zhao & Ying Ye & Shaoping Zhang & Xudong Huang & Ruihong Bai & Lisha Zhuang & Quanbo Zhou & Mei Li & Jiachun Su & Rui Li & Xiaoqiong Bao & Lingxing Z, 2023. "CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:385:y:1997:i:6614:d:10.1038_385357a0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.