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Subcellular connectomic analyses of energy networks in striated muscle

Author

Listed:
  • Christopher K. E. Bleck

    (National Institutes of Health)

  • Yuho Kim

    (National Institutes of Health)

  • T. Bradley Willingham

    (National Institutes of Health)

  • Brian Glancy

    (National Institutes of Health
    National Institutes of Health)

Abstract

Mapping biological circuit connectivity has revolutionized our understanding of structure-function relationships. Although connectomic analyses have primarily focused on neural systems, electrical connectivity within muscle mitochondrial networks was recently demonstrated to provide a rapid mechanism for cellular energy distribution. However, tools to evaluate organelle connectivity with high spatial fidelity within single cells are currently lacking. Here, we developed a framework to quantitatively assess mitochondrial network connectivity and interactions with cellular sites of energy storage, utilization, and calcium cycling in cardiac, oxidative, and glycolytic muscle. We demonstrate that mitochondrial network configuration, individual mitochondrial size and shape, and the junctions connecting mitochondria within each network are consistent with the differing contraction demands of each muscle type. Moreover, mitochondria-lipid droplet interaction analyses suggest that individual mitochondria within networks may play specialized roles regarding energy distribution and calcium cycling within the cell and reveal the power of connectomic analyses of organelle interactions within single cells.

Suggested Citation

  • Christopher K. E. Bleck & Yuho Kim & T. Bradley Willingham & Brian Glancy, 2018. "Subcellular connectomic analyses of energy networks in striated muscle," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07676-y
    DOI: 10.1038/s41467-018-07676-y
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    Cited by:

    1. Prasanna Katti & Alexander S. Hall & Hailey A. Parry & Peter T. Ajayi & Yuho Kim & T. Bradley Willingham & Christopher K. E. Bleck & Han Wen & Brian Glancy, 2022. "Mitochondrial network configuration influences sarcomere and myosin filament structure in striated muscles," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Peter T. Ajayi & Prasanna Katti & Yingfan Zhang & T. Bradley Willingham & Ye Sun & Christopher K. E. Bleck & Brian Glancy, 2022. "Regulation of the evolutionarily conserved muscle myofibrillar matrix by cell type dependent and independent mechanisms," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Prasanna Katti & Peter T. Ajayi & Angel Aponte & Christopher K. E. Bleck & Brian Glancy, 2022. "Identification of evolutionarily conserved regulators of muscle mitochondrial network organization," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    4. Gabriela Poliacikova & Marine Barthez & Thomas Rival & Aïcha Aouane & Nuno Miguel Luis & Fabrice Richard & Fabrice Daian & Nicolas Brouilly & Frank Schnorrer & Corinne Maurel-Zaffran & Yacine Graba & , 2023. "M1BP is an essential transcriptional activator of oxidative metabolism during Drosophila development," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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