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Compartmentalised RNA catalysis in membrane-free coacervate protocells

Author

Listed:
  • Björn Drobot

    (Max-Planck Institute for Molecular Cell Biology and Genetics)

  • Juan M. Iglesias-Artola

    (Max-Planck Institute for Molecular Cell Biology and Genetics)

  • Kristian Vay

    (Max-Planck Institute for Biochemistry)

  • Viktoria Mayr

    (Max-Planck Institute for Biochemistry)

  • Mrityunjoy Kar

    (Max-Planck Institute for Molecular Cell Biology and Genetics)

  • Moritz Kreysing

    (Max-Planck Institute for Molecular Cell Biology and Genetics)

  • Hannes Mutschler

    (Max-Planck Institute for Biochemistry)

  • T-Y Dora Tang

    (Max-Planck Institute for Molecular Cell Biology and Genetics)

Abstract

Phase separation of mixtures of oppositely charged polymers provides a simple and direct route to compartmentalisation via complex coacervation, which may have been important for driving primitive reactions as part of the RNA world hypothesis. However, to date, RNA catalysis has not been reconciled with coacervation. Here we demonstrate that RNA catalysis is viable within coacervate microdroplets and further show that these membrane-free droplets can selectively retain longer length RNAs while permitting transfer of lower molecular weight oligonucleotides.

Suggested Citation

  • Björn Drobot & Juan M. Iglesias-Artola & Kristian Vay & Viktoria Mayr & Mrityunjoy Kar & Moritz Kreysing & Hannes Mutschler & T-Y Dora Tang, 2018. "Compartmentalised RNA catalysis in membrane-free coacervate protocells," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06072-w
    DOI: 10.1038/s41467-018-06072-w
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    Cited by:

    1. Cheng Qi & Xudong Ma & Qi Zeng & Zhangwei Huang & Shanshan Zhang & Xiaokang Deng & Tiantian Kong & Zhou Liu, 2024. "Multicompartmental coacervate-based protocell by spontaneous droplet evaporation," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    2. Tommaso P. Fraccia & Nicolas Martin, 2023. "Non-enzymatic oligonucleotide ligation in coacervate protocells sustains compartment-content coupling," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Shoupeng Cao & Tsvetomir Ivanov & Julian Heuer & Calum T. J. Ferguson & Katharina Landfester & Lucas Caire da Silva, 2024. "Dipeptide coacervates as artificial membraneless organelles for bioorthogonal catalysis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    4. Elia Salibi & Benedikt Peter & Petra Schwille & Hannes Mutschler, 2023. "Periodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populations," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    5. Annalena Salditt & Leonie Karr & Elia Salibi & Kristian Vay & Dieter Braun & Hannes Mutschler, 2023. "Ribozyme-mediated RNA synthesis and replication in a model Hadean microenvironment," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    6. Vincent Ouazan-Reboul & Jaime Agudo-Canalejo & Ramin Golestanian, 2023. "Self-organization of primitive metabolic cycles due to non-reciprocal interactions," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    7. Jiahua Wang & Manzar Abbas & Junyou Wang & Evan Spruijt, 2023. "Selective amide bond formation in redox-active coacervate protocells," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    8. Peiying Li & Philipp Holliger & Shunsuke Tagami, 2022. "Hydrophobic-cationic peptides modulate RNA polymerase ribozyme activity by accretion," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    9. Alexander M. Bergmann & Jonathan Bauermann & Giacomo Bartolucci & Carsten Donau & Michele Stasi & Anna-Lena Holtmannspötter & Frank Jülicher & Christoph A. Weber & Job Boekhoven, 2023. "Liquid spherical shells are a non-equilibrium steady state of active droplets," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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