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Region and cell-type resolved quantitative proteomic map of the human heart

Author

Listed:
  • Sophia Doll

    (Max Planck Institute of Biochemistry
    Faculty of Health Sciences, University of Copenhagen)

  • Martina Dreßen

    (German Heart Center Munich at the Technische Universität München)

  • Philipp E. Geyer

    (Max Planck Institute of Biochemistry
    Faculty of Health Sciences, University of Copenhagen)

  • Daniel N. Itzhak

    (Max Planck Institute of Biochemistry)

  • Christian Braun

    (Ludwig-Maximilians-University)

  • Stefanie A. Doppler

    (German Heart Center Munich at the Technische Universität München)

  • Florian Meier

    (Max Planck Institute of Biochemistry)

  • Marcus-Andre Deutsch

    (German Heart Center Munich at the Technische Universität München
    Partner Site Munich Heart Alliance)

  • Harald Lahm

    (German Heart Center Munich at the Technische Universität München)

  • Rüdiger Lange

    (German Heart Center Munich at the Technische Universität München
    Partner Site Munich Heart Alliance)

  • Markus Krane

    (German Heart Center Munich at the Technische Universität München
    Partner Site Munich Heart Alliance)

  • Matthias Mann

    (Max Planck Institute of Biochemistry
    Faculty of Health Sciences, University of Copenhagen)

Abstract

The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.

Suggested Citation

  • Sophia Doll & Martina Dreßen & Philipp E. Geyer & Daniel N. Itzhak & Christian Braun & Stefanie A. Doppler & Florian Meier & Marcus-Andre Deutsch & Harald Lahm & Rüdiger Lange & Markus Krane & Matthia, 2017. "Region and cell-type resolved quantitative proteomic map of the human heart," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01747-2
    DOI: 10.1038/s41467-017-01747-2
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    Cited by:

    1. Jingbo Qie & Yang Liu & Yunzhi Wang & Fan Zhang & Zhaoyu Qin & Sha Tian & Mingwei Liu & Kai Li & Wenhao Shi & Lei Song & Mingjun Sun & Yexin Tong & Ping Hu & Tao Gong & Xiaqiong Wang & Yi Huang & Bolo, 2022. "Integrated proteomic and transcriptomic landscape of macrophages in mouse tissues," Nature Communications, Nature, vol. 13(1), pages 1-23, December.
    2. Tirthankar Mohanty & Christofer A. Q. Karlsson & Yashuan Chao & Erik Malmström & Eleni Bratanis & Andrietta Grentzmann & Martina Mørch & Victor Nizet & Lars Malmström & Adam Linder & Oonagh Shannon & , 2023. "A pharmacoproteomic landscape of organotypic intervention responses in Gram-negative sepsis," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Zongyuan Liu & Rebecca Ulrich vonBargen & April L. Kendricks & Kate Wheeler & Ana Carolina Leão & Krithivasan Sankaranarayanan & Danya A. Dean & Shelley S. Kane & Ekram Hossain & Jeroen Pollet & Maria, 2023. "Localized cardiac small molecule trajectories and persistent chemical sequelae in experimental Chagas disease," Nature Communications, Nature, vol. 14(1), pages 1-22, December.

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