IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms6846.html
   My bibliography  Save this article

Filopodia-based Wnt transport during vertebrate tissue patterning

Author

Listed:
  • Eliana Stanganello

    (Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1)

  • Anja I. H. Hagemann

    (Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1)

  • Benjamin Mattes

    (Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1)

  • Claude Sinner

    (Steinbuch Center for Computing (SCC), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1
    Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1)

  • Dana Meyen

    (Institute of Cell biology, ZMBE, University of Münster)

  • Sabrina Weber

    (Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1)

  • Alexander Schug

    (Steinbuch Center for Computing (SCC), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1)

  • Erez Raz

    (Institute of Cell biology, ZMBE, University of Münster)

  • Steffen Scholpp

    (Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1)

Abstract

Paracrine Wnt/β-catenin signalling is important during developmental processes, tissue regeneration and stem cell regulation. Wnt proteins are morphogens, which form concentration gradients across responsive tissues. Little is known about the transport mechanism for these lipid-modified signalling proteins in vertebrates. Here we show that Wnt8a is transported on actin-based filopodia to contact responding cells and activate signalling during neural plate formation in zebrafish. Cdc42/N-Wasp regulates the formation of these Wnt-positive filopodia. Enhanced formation of filopodia increases the effective signalling range of Wnt by facilitating spreading. Consistently, reduction in filopodia leads to a restricted distribution of the ligand and a limited signalling range. Using a simulation, we provide evidence that such a short-range transport system for Wnt has a long-range signalling function. Indeed, we show that a filopodia-based transport system for Wnt8a controls anteroposterior patterning of the neural plate during vertebrate gastrulation.

Suggested Citation

  • Eliana Stanganello & Anja I. H. Hagemann & Benjamin Mattes & Claude Sinner & Dana Meyen & Sabrina Weber & Alexander Schug & Erez Raz & Steffen Scholpp, 2015. "Filopodia-based Wnt transport during vertebrate tissue patterning," Nature Communications, Nature, vol. 6(1), pages 1-14, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6846
    DOI: 10.1038/ncomms6846
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms6846
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms6846?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Adrián Aguirre-Tamaral & Manuel Cambón & David Poyato & Juan Soler & Isabel Guerrero, 2022. "Predictive model for cytoneme guidance in Hedgehog signaling based on Ihog- Glypicans interaction," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Lijuan Du & Alex Sohr & Yujia Li & Sougata Roy, 2022. "GPI-anchored FGF directs cytoneme-mediated bidirectional contacts to regulate its tissue-specific dispersion," Nature Communications, Nature, vol. 13(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6846. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.