IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms6277.html
   My bibliography  Save this article

MS-GF+ makes progress towards a universal database search tool for proteomics

Author

Listed:
  • Sangtae Kim

    (University of California San Diego, La Jolla, California 92093, USA
    Present address: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA)

  • Pavel A. Pevzner

    (University of California San Diego, La Jolla, California 92093, USA)

Abstract

Mass spectrometry (MS) instruments and experimental protocols are rapidly advancing, but the software tools to analyse tandem mass spectra are lagging behind. We present a database search tool MS-GF+ that is sensitive (it identifies more peptides than most other database search tools) and universal (it works well for diverse types of spectra, different configurations of MS instruments and different experimental protocols). We benchmark MS-GF+ using diverse spectral data sets: (i) spectra of varying fragmentation methods; (ii) spectra of multiple enzyme digests; (iii) spectra of phosphorylated peptides; and (iv) spectra of peptides with unusual fragmentation propensities produced by a novel alpha-lytic protease. For all these data sets, MS-GF+ significantly increases the number of identified peptides compared with commonly used methods for peptide identifications. We emphasize that although MS-GF+ is not specifically designed for any particular experimental set-up, it improves on the performance of tools specifically designed for these applications (for example, specialized tools for phosphoproteomics).

Suggested Citation

  • Sangtae Kim & Pavel A. Pevzner, 2014. "MS-GF+ makes progress towards a universal database search tool for proteomics," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6277
    DOI: 10.1038/ncomms6277
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms6277
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/ncomms6277?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Zheng Fang & Hongqiang Qin & Jiawei Mao & Zhongyu Wang & Na Zhang & Yan Wang & Luyao Liu & Yongzhan Nie & Mingming Dong & Mingliang Ye, 2022. "Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Kaiyuan Liu & Yuzhen Ye & Sujun Li & Haixu Tang, 2023. "Accurate de novo peptide sequencing using fully convolutional neural networks," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Rani Zananiri & Sivasubramanyan Mangapuram Venkata & Vera Gaydar & Dan Yahalom & Omri Malik & Sergei Rudnizky & Oded Kleifeld & Ariel Kaplan & Arnon Henn, 2022. "Auxiliary ATP binding sites support DNA unwinding by RecBCD," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    4. Isabelle Rose Leo & Luay Aswad & Matthias Stahl & Elena Kunold & Frederik Post & Tom Erkers & Nona Struyf & Georgios Mermelekas & Rubin Narayan Joshi & Eva Gracia-Villacampa & Päivi Östling & Olli P. , 2022. "Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    5. Guanlan Xu & Tiffany D. Grimes & Truman B. Grayson & Junqin Chen & Lance A. Thielen & Hubert M. Tse & Peng Li & Matt Kanke & Tai-Tu Lin & Athena A. Schepmoes & Adam C. Swensen & Vladislav A. Petyuk & , 2022. "Exploratory study reveals far reaching systemic and cellular effects of verapamil treatment in subjects with type 1 diabetes," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    6. Haris Babačić & Wanda Christ & José Eduardo Araújo & Georgios Mermelekas & Nidhi Sharma & Janne Tynell & Marina García & Renata Varnaite & Hilmir Asgeirsson & Hedvig Glans & Janne Lehtiö & Sara Gredma, 2023. "Comprehensive proteomics and meta-analysis of COVID-19 host response," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    7. Bo Wen & Bing Zhang, 2023. "PepQuery2 democratizes public MS proteomics data for rapid peptide searching," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    8. Jong Woo Bae & Sangtae Kim & V. Narry Kim & Jong-Seo Kim, 2021. "Photoactivatable ribonucleosides mark base-specific RNA-binding sites," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    9. Heather Schiller & Yirui Hong & Joshua Kouassi & Theopi Rados & Jasmin Kwak & Anthony DiLucido & Daniel Safer & Anita Marchfelder & Friedhelm Pfeiffer & Alexandre Bisson & Stefan Schulze & Mechthild P, 2024. "Identification of structural and regulatory cell-shape determinants in Haloferax volcanii," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    10. Robert H. Lampe & Tyler H. Coale & Kiefer O. Forsch & Loay J. Jabre & Samuel Kekuewa & Erin M. Bertrand & Aleš Horák & Miroslav Oborník & Ariel J. Rabines & Elden Rowland & Hong Zheng & Andreas J. And, 2023. "Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6277. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.