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Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis

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  • Charlotte H. Coles

    (Wellcome Trust Centre for Human Genetics, University of Oxford
    Present address: Laboratory for Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), Ludwig‐Erhard‐Allee 2, 53175 Bonn, Germany)

  • Nikolaos Mitakidis

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Peng Zhang

    (University of British Columbia)

  • Jonathan Elegheert

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Weixian Lu

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Andrew W. Stoker

    (Cancer Section, Institute of Child Health, University College London)

  • Terunaga Nakagawa

    (Vanderbilt University, School of Medicine)

  • Ann Marie Craig

    (University of British Columbia)

  • E. Yvonne Jones

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • A. Radu Aricescu

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

Abstract

Receptor protein tyrosine phosphatase sigma (RPTPσ) regulates neuronal extension and acts as a presynaptic nexus for multiple protein and proteoglycan interactions during synaptogenesis. Unknown mechanisms govern the shift in RPTPσ function, from outgrowth promotion to synaptic organization. Here, we report crystallographic, electron microscopic and small-angle X-ray scattering analyses, which reveal sufficient inter-domain flexibility in the RPTPσ extracellular region for interaction with both cis (same cell) and trans (opposite cell) ligands. Crystal structures of RPTPσ bound to its postsynaptic ligand TrkC detail an interaction surface partially overlapping the glycosaminoglycan-binding site. Accordingly, heparan sulphate and heparin oligomers compete with TrkC for RPTPσ binding in vitro and disrupt TrkC-dependent synaptic differentiation in neuronal co-culture assays. We propose that transient RPTPσ ectodomain emergence from the presynaptic proteoglycan layer allows capture by TrkC to form a trans-synaptic complex, the consequent reduction in RPTPσ flexibility potentiating interactions with additional ligands to orchestrate excitatory synapse formation.

Suggested Citation

  • Charlotte H. Coles & Nikolaos Mitakidis & Peng Zhang & Jonathan Elegheert & Weixian Lu & Andrew W. Stoker & Terunaga Nakagawa & Ann Marie Craig & E. Yvonne Jones & A. Radu Aricescu, 2014. "Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis," Nature Communications, Nature, vol. 5(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6209
    DOI: 10.1038/ncomms6209
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    1. F. M. Houghton & S. E. Adams & A. S. Ríos & L. Masino & A. G. Purkiss & D. C. Briggs & F. Ledda & N. Q. McDonald, 2023. "Architecture and regulation of a GDNF-GFRα1 synaptic adhesion assembly," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Christos Gogou & J. Wouter Beugelink & Cátia P. Frias & Leanid Kresik & Natalia Jaroszynska & Uwe Drescher & Bert J. C. Janssen & Robert Hindges & Dimphna H. Meijer, 2024. "Alternative splicing controls teneurin-3 compact dimer formation for neuronal recognition," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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