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Self-luminescing BRET-FRET near-infrared dots for in vivo lymph-node mapping and tumour imaging

Author

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  • Liqin Xiong

    (Molecular Imaging Program at Stanford (MIPS), Stanford University
    Present address: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China)

  • Adam J. Shuhendler

    (Molecular Imaging Program at Stanford (MIPS), Stanford University)

  • Jianghong Rao

    (Molecular Imaging Program at Stanford (MIPS), Stanford University)

Abstract

Strong autofluorescence from living tissues, and the scattering and absorption of short-wavelength light in living tissues, significantly reduce sensitivity of in vivo fluorescence imaging. These issues can be tackled by using imaging probes that emit in the near-infrared wavelength range. Here we describe self-luminescing near-infrared-emitting nanoparticles employing an energy transfer relay that integrates bioluminescence resonance energy transfer and fluorescence resonance energy transfer, enabling in vivo near-infrared imaging without external light excitation. Nanoparticles were 30–40 nm in diameter, contained no toxic metals, exhibited long circulation time and high serum stability, and produced strong near-infrared emission. Using these nanoparticles, we successfully imaged lymphatic networks and vasculature of xenografted tumours in living mice. The self-luminescing feature provided excellent tumour-to-background ratio (>100) for imaging very small tumours (2–3 mm in diameter). Our results demonstrate that these new nanoparticles are well suited to in vivo imaging applications such as lymph-node mapping and cancer imaging.

Suggested Citation

  • Liqin Xiong & Adam J. Shuhendler & Jianghong Rao, 2012. "Self-luminescing BRET-FRET near-infrared dots for in vivo lymph-node mapping and tumour imaging," Nature Communications, Nature, vol. 3(1), pages 1-9, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms2197
    DOI: 10.1038/ncomms2197
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    1. Qian Zhang & Bin Song & Yanan Xu & Yunmin Yang & Jian Ji & Wenjun Cao & Jianping Lu & Jiali Ding & Haiting Cao & Binbin Chu & Jiaxu Hong & Houyu Wang & Yao He, 2023. "In vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Luyan Wu & Yusuke Ishigaki & Wenhui Zeng & Takashi Harimoto & Baoli Yin & Yinghan Chen & Shiyi Liao & Yongchun Liu & Yidan Sun & Xiaobo Zhang & Ying Liu & Yong Liang & Pengfei Sun & Takanori Suzuki & , 2021. "Generation of hydroxyl radical-activatable ratiometric near-infrared bimodal probes for early monitoring of tumor response to therapy," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

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