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A distinct double-ring LH1–LH2 photocomplex from an extremophilic phototroph

Author

Listed:
  • Kazutoshi Tani

    (University of Tsukuba
    Mie University)

  • Kenji V. P. Nagashima

    (Kanagawa University)

  • Risa Kojima

    (Ritsumeikan University)

  • Masaharu Kondo

    (Nagoya Institute of Technology)

  • Ryo Kanno

    (Okinawa Institute of Science and Technology Graduate University (OIST))

  • Issei Satoh

    (Ibaraki University)

  • Mai Kawakami

    (Ibaraki University)

  • Naho Hiwatashi

    (Ibaraki University)

  • Kazuna Nakata

    (Kobe University)

  • Sakiko Nagashima

    (Kanagawa University)

  • Kazuhito Inoue

    (Kanagawa University
    Kanagawa University)

  • Yugo Isawa

    (Ritsumeikan University)

  • Ryoga Morishita

    (Nagoya Institute of Technology)

  • Shinichi Takaichi

    (Tokyo University of Agriculture)

  • Endang R. Purba

    (Okinawa Institute of Science and Technology Graduate University (OIST))

  • Malgorzata Hall

    (Okinawa Institute of Science and Technology Graduate University (OIST))

  • Long-Jiang Yu

    (Chinese Academy of Sciences)

  • Michael T. Madigan

    (Southern Illinois University)

  • Akira Mizoguchi

    (Mie University)

  • Bruno M. Humbel

    (Okinawa Institute of Science and Technology Graduate University (OIST)
    Juntendo University)

  • Yukihiro Kimura

    (Kobe University)

  • Yutaka Nagasawa

    (Ritsumeikan University)

  • Takehisa Dewa

    (Nagoya Institute of Technology)

  • Zheng-Yu Wang-Otomo

    (Ibaraki University)

Abstract

Halorhodospira (Hlr.) halophila strain BN9622 is an extremely halophilic and alkaliphilic phototrophic purple sulfur bacterium isolated from a hypersaline lake in the Libyan Desert whose total salinity exceeded 35% at pH 10.7. Here we present a cryo-EM structure of the native LH1–LH2 co-complex from strain BN9622 at 2.22 Å resolution. Surprisingly, the LH1–LH2 co-complex consists of a double-ring cylindrical structure with the larger LH1 ring encircling a smaller LH2 ring. The Hlr. halophila LH1 contains 18 αβ-subunits and additional bacteriochlorophyll a (BChl a) molecules that absorb maximally at 797 nm. The LH2 ring is composed of 9 αβ-subunits, and the BChl a molecules in the co-complex form extensive intra- and inter-complex networks to allow near 100% efficiency of energy transfer to its surrounding LH1. The additional LH1-B797 BChls a are located in such a manner that they facilitate exciton transfer from monomeric BChls in LH2 to the dimeric BChls in LH1. The structural features of the strain BN9622 LH1–LH2 co-complex may have evolved to allow a minimal LH2 complex to maximize excitation transfer to the core complex and effectively harvest light in the physiologically demanding ecological niche of this purple bacterium.

Suggested Citation

  • Kazutoshi Tani & Kenji V. P. Nagashima & Risa Kojima & Masaharu Kondo & Ryo Kanno & Issei Satoh & Mai Kawakami & Naho Hiwatashi & Kazuna Nakata & Sakiko Nagashima & Kazuhito Inoue & Yugo Isawa & Ryoga, 2025. "A distinct double-ring LH1–LH2 photocomplex from an extremophilic phototroph," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-55811-9
    DOI: 10.1038/s41467-024-55811-9
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    References listed on IDEAS

    as
    1. Yueyong Xin & Yang Shi & Tongxin Niu & Qingqiang Wang & Wanqiang Niu & Xiaojun Huang & Wei Ding & Lei Yang & Robert E. Blankenship & Xiaoling Xu & Fei Sun, 2018. "Cryo-EM structure of the RC-LH core complex from an early branching photosynthetic prokaryote," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    2. Pu Qian & C. Alistair Siebert & Peiyi Wang & Daniel P. Canniffe & C. Neil Hunter, 2018. "Cryo-EM structure of the Blastochloris viridis LH1–RC complex at 2.9 Å," Nature, Nature, vol. 556(7700), pages 203-208, April.
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