IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-17606-6.html
   My bibliography  Save this article

Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP

Author

Listed:
  • Estelle Crozat

    (Université de Toulouse, CNRS, UPS
    University of Cambridge)

  • Catherine Tardin

    (Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS)

  • Maya Salhi

    (Université de Toulouse, CNRS, UPS)

  • Philippe Rousseau

    (Université de Toulouse, CNRS, UPS)

  • Armand Lablaine

    (Université de Toulouse, CNRS, UPS)

  • Tommaso Bertoni

    (University of Cambridge)

  • David Holcman

    (Ecole Normale Supérieure, Applied Math and Computational Biology, IBENS)

  • Bianca Sclavi

    (Sorbonne Université)

  • Pietro Cicuta

    (University of Cambridge)

  • François Cornet

    (Université de Toulouse, CNRS, UPS)

Abstract

The ter region of the bacterial chromosome, where replication terminates, is the last to be segregated before cell division in Escherichia coli. Delayed segregation is controlled by the MatP protein, which binds to specific sites (matS) within ter, and interacts with other proteins such as ZapB. Here, we investigate the role of MatP by combining short-time mobility analyses of the ter locus with biochemical approaches. We find that ter mobility is similar to that of a non ter locus, except when sister ter loci are paired after replication. This effect depends on MatP, the persistence of catenanes, and ZapB. We characterise MatP/DNA complexes and conclude that MatP binds DNA as a tetramer, but bridging matS sites in a DNA-rich environment remains infrequent. We propose that tetramerisation of MatP links matS sites with ZapB and/or with non-specific DNA to promote optimal pairing of sister ter regions until cell division.

Suggested Citation

  • Estelle Crozat & Catherine Tardin & Maya Salhi & Philippe Rousseau & Armand Lablaine & Tommaso Bertoni & David Holcman & Bianca Sclavi & Pietro Cicuta & François Cornet, 2020. "Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17606-6
    DOI: 10.1038/s41467-020-17606-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-17606-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-17606-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ismath Sadhir & Seán M. Murray, 2023. "Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17606-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.