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Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP

Author

Listed:
  • Estelle Crozat

    (Université de Toulouse, CNRS, UPS
    University of Cambridge)

  • Catherine Tardin

    (Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS)

  • Maya Salhi

    (Université de Toulouse, CNRS, UPS)

  • Philippe Rousseau

    (Université de Toulouse, CNRS, UPS)

  • Armand Lablaine

    (Université de Toulouse, CNRS, UPS)

  • Tommaso Bertoni

    (University of Cambridge)

  • David Holcman

    (Ecole Normale Supérieure, Applied Math and Computational Biology, IBENS)

  • Bianca Sclavi

    (Sorbonne Université)

  • Pietro Cicuta

    (University of Cambridge)

  • François Cornet

    (Université de Toulouse, CNRS, UPS)

Abstract

The ter region of the bacterial chromosome, where replication terminates, is the last to be segregated before cell division in Escherichia coli. Delayed segregation is controlled by the MatP protein, which binds to specific sites (matS) within ter, and interacts with other proteins such as ZapB. Here, we investigate the role of MatP by combining short-time mobility analyses of the ter locus with biochemical approaches. We find that ter mobility is similar to that of a non ter locus, except when sister ter loci are paired after replication. This effect depends on MatP, the persistence of catenanes, and ZapB. We characterise MatP/DNA complexes and conclude that MatP binds DNA as a tetramer, but bridging matS sites in a DNA-rich environment remains infrequent. We propose that tetramerisation of MatP links matS sites with ZapB and/or with non-specific DNA to promote optimal pairing of sister ter regions until cell division.

Suggested Citation

  • Estelle Crozat & Catherine Tardin & Maya Salhi & Philippe Rousseau & Armand Lablaine & Tommaso Bertoni & David Holcman & Bianca Sclavi & Pietro Cicuta & François Cornet, 2020. "Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17606-6
    DOI: 10.1038/s41467-020-17606-6
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    Cited by:

    1. Ismath Sadhir & Seán M. Murray, 2023. "Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Elena Espinosa & Jihane Challita & Jean-Michel Desfontaines & Christophe Possoz & Marie-Eve Val & Didier Mazel & Martial Marbouty & Romain Koszul & Elisa Galli & François-Xavier Barre, 2024. "MatP local enrichment delays segregation independently of tetramer formation and septal anchoring in Vibrio cholerae," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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