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HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2

Author

Listed:
  • Venecia Alexandria Valdez

    (Princeton University)

  • Meisheng Ma

    (School of Medicine
    Huazhong University of Science and Technology)

  • Bernardo Gouveia

    (Princeton University)

  • Rui Zhang

    (School of Medicine)

  • Sabine Petry

    (Princeton University)

Abstract

In vertebrate spindles, most microtubules are formed via branching microtubule nucleation, whereby microtubules nucleate along the side of pre-existing microtubules. Hepatoma up-regulated protein (HURP) is a microtubule-associated protein that has been implicated in spindle assembly, but its mode of action is yet to be defined. In this study, we show that HURP is necessary for RanGTP-induced branching microtubule nucleation in Xenopus egg extract. Specifically, HURP stabilizes the microtubule lattice to promote microtubule formation from γ-TuRC. This function is shifted to promote branching microtubule nucleation through enhanced localization to TPX2 condensates, which form the core of the branch site on microtubules. Lastly, we provide a high-resolution cryo-EM structure of HURP on the microtubule, revealing how HURP binding stabilizes the microtubule lattice. We propose a model in which HURP stabilizes microtubules during their formation, and TPX2 preferentially enriches HURP to microtubules to promote branching microtubule nucleation and thus spindle assembly.

Suggested Citation

  • Venecia Alexandria Valdez & Meisheng Ma & Bernardo Gouveia & Rui Zhang & Sabine Petry, 2024. "HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53630-6
    DOI: 10.1038/s41467-024-53630-6
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    References listed on IDEAS

    as
    1. Bernardo Gouveia & Sagar U. Setru & Matthew R. King & Aaron Hamlin & Howard A. Stone & Joshua W. Shaevitz & Sabine Petry, 2023. "Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes in Xenopus laevis egg extract," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Sophie M. Travis & Brian P. Mahon & Wei Huang & Meisheng Ma & Michael J. Rale & Jodi Kraus & Derek J. Taylor & Rui Zhang & Sabine Petry, 2023. "Integrated model of the vertebrate augmin complex," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    3. Matthew R. King & Sabine Petry, 2020. "Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    4. Cédric Castrogiovanni & Alessio V. Inchingolo & Jonathan U. Harrison & Damian Dudka & Onur Sen & Nigel J. Burroughs & Andrew D. McAinsh & Patrick Meraldi, 2022. "Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
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