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Chemoproteogenomic stratification of the missense variant cysteinome

Author

Listed:
  • Heta Desai

    (UCLA
    UCLA)

  • Katrina H. Andrews

    (UCLA)

  • Kristina V. Bergersen

    (UCLA)

  • Samuel Ofori

    (UCLA)

  • Fengchao Yu

    (University of Michigan)

  • Flowreen Shikwana

    (UCLA
    UCLA)

  • Mark A. Arbing

    (UCLA
    UCLA)

  • Lisa M. Boatner

    (UCLA
    UCLA)

  • Miranda Villanueva

    (UCLA
    UCLA)

  • Nicholas Ung

    (UCLA)

  • Elaine F. Reed

    (UCLA)

  • Alexey I. Nesvizhskii

    (University of Michigan
    University of Michigan)

  • Keriann M. Backus

    (UCLA
    UCLA
    UCLA
    UCLA)

Abstract

Cancer genomes are rife with genetic variants; one key outcome of this variation is widespread gain-of-cysteine mutations. These acquired cysteines can be both driver mutations and sites targeted by precision therapies. However, despite their ubiquity, nearly all acquired cysteines remain unidentified via chemoproteomics; identification is a critical step to enable functional analysis, including assessment of potential druggability and susceptibility to oxidation. Here, we pair cysteine chemoproteomics—a technique that enables proteome-wide pinpointing of functional, redox sensitive, and potentially druggable residues—with genomics to reveal the hidden landscape of cysteine genetic variation. Our chemoproteogenomics platform integrates chemoproteomic, whole exome, and RNA-seq data, with a customized two-stage false discovery rate (FDR) error controlled proteomic search, which is further enhanced with a user-friendly FragPipe interface. Chemoproteogenomics analysis reveals that cysteine acquisition is a ubiquitous feature of both healthy and cancer genomes that is further elevated in the context of decreased DNA repair. Reference cysteines proximal to missense variants are also found to be pervasive, supporting heretofore untapped opportunities for variant-specific chemical probe development campaigns. As chemoproteogenomics is further distinguished by sample-matched combinatorial variant databases and is compatible with redox proteomics and small molecule screening, we expect widespread utility in guiding proteoform-specific biology and therapeutic discovery.

Suggested Citation

  • Heta Desai & Katrina H. Andrews & Kristina V. Bergersen & Samuel Ofori & Fengchao Yu & Flowreen Shikwana & Mark A. Arbing & Lisa M. Boatner & Miranda Villanueva & Nicholas Ung & Elaine F. Reed & Alexe, 2024. "Chemoproteogenomic stratification of the missense variant cysteinome," Nature Communications, Nature, vol. 15(1), pages 1-24, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53520-x
    DOI: 10.1038/s41467-024-53520-x
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