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IL-1β-induced epithelial cell and fibroblast transdifferentiation promotes neutrophil recruitment in chronic rhinosinusitis with nasal polyps

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  • Xinyu Xie

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

  • Pin Wang

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

  • Min Jin

    (Qilu Hospital of Shandong University)

  • Yue Wang

    (Qilu Hospital of Shandong University)

  • Lijie Qi

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

  • Changhua Wu

    (Qilu Hospital of Shandong University)

  • Shu Guo

    (Qilu Hospital of Shandong University)

  • Changqing Li

    (Qilu Hospital of Shandong University)

  • Xiaojun Zhang

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

  • Ye Yuan

    (Qilu Hospital of Shandong University)

  • Xinyi Ma

    (Qilu Hospital of Shandong University)

  • Fangying Liu

    (Qilu Hospital of Shandong University)

  • Weiyuan Liu

    (Qilu Hospital of Shandong University)

  • Heng Liu

    (Qilu Hospital of Shandong University)

  • Chen Duan

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

  • Ping Ye

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

  • Xuezhong Li

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

  • Larry Borish

    (University of Virginia Health System
    University of Virginia Health System)

  • Wei Zhao

    (Shandong University
    Shandong University)

  • Xin Feng

    (Qilu Hospital of Shandong University
    Qilu Hospital of Shandong University)

Abstract

Neutrophilic inflammation contributes to multiple chronic inflammatory airway diseases, including asthma and chronic rhinosinusitis with nasal polyps (CRSwNP), and is associated with an unfavorable prognosis. Here, using single-cell RNA sequencing (scRNA-seq) to profile human nasal mucosa obtained from the inferior turbinates, middle turbinates, and nasal polyps of CRSwNP patients, we identify two IL-1 signaling-induced cell subsets—LY6D+ club cells and IDO1+ fibroblasts—that promote neutrophil recruitment by respectively releasing S100A8/A9 and CXCL1/2/3/5/6/8 into inflammatory regions. IL-1β, a pro-inflammatory cytokine involved in IL-1 signaling, induces the transdifferentiation of LY6D+ club cells and IDO1+ fibroblasts from primary epithelial cells and fibroblasts, respectively. In an LPS-induced neutrophilic CRSwNP mouse model, blocking IL-1β activity with a receptor antagonist significantly reduces the numbers of LY6D+ club cells and IDO1+ fibroblasts and mitigates nasal inflammation. This study implicates the function of two cell subsets in neutrophil recruitment and demonstrates an IL-1-based intervention for mitigating neutrophilic inflammation in CRSwNP.

Suggested Citation

  • Xinyu Xie & Pin Wang & Min Jin & Yue Wang & Lijie Qi & Changhua Wu & Shu Guo & Changqing Li & Xiaojun Zhang & Ye Yuan & Xinyi Ma & Fangying Liu & Weiyuan Liu & Heng Liu & Chen Duan & Ping Ye & Xuezhon, 2024. "IL-1β-induced epithelial cell and fibroblast transdifferentiation promotes neutrophil recruitment in chronic rhinosinusitis with nasal polyps," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53307-0
    DOI: 10.1038/s41467-024-53307-0
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