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Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients

Author

Listed:
  • Na Chen

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Lilan Hao

    (BGI-Research
    University of Copenhagen
    BGI-Research)

  • Zhe Zhang

    (BGI-Research)

  • Chenglu Qin

    (The 3rd Affiliated Hospital of Shenzhen University, Luohu hospital)

  • Zhuye Jie

    (BGI-Research
    University of Copenhagen)

  • Hongxin Pan

    (Southern University of Science and Technology Hospital)

  • Jiali Duan

    (4+4 Medical Doctor Program, No.9 Dongdansantiao)

  • Xincheng Huang

    (BGI-Research
    BGI-Research)

  • Yunhong Zhang

    (Social Affairs Bureau of Suzhou National New and Hi-tech Industrial Development Zone)

  • Hongqin Gao

    (Suzhou National New and Hi-tech Industrial Development Zone Center for Maternal and Child Health and Family Planning Service)

  • Ruike Lu

    (Suzhou National New and Hi-tech Industrial Development Zone Center for Maternal and Child Health and Family Planning Service)

  • Tianshu Sun

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Hua Yang

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Jinqiu Shi

    (The 3rd Affiliated Hospital of Shenzhen University, Luohu hospital)

  • Maolian Liang

    (The 3rd Affiliated Hospital of Shenzhen University, Luohu hospital)

  • Jianbin Guo

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Qianqian Gao

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Xiaoyue Zhao

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Zhiyuan Dou

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Liang Xiao

    (BGI-Research
    BGI-Research
    BGI-Research)

  • Shaoqiao Zhang

    (BGI Research)

  • Xin Jin

    (BGI-Research)

  • Xun Xu

    (BGI-Research)

  • Huanming Yang

    (BGI-Research
    James D. Watson Institute of Genome Sciences)

  • Jian Wang

    (BGI-Research
    James D. Watson Institute of Genome Sciences)

  • Huijue Jia

    (BGI-Research
    Fudan University
    Fudan University)

  • Tao Zhang

    (BGI Research
    Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research)

  • Karsten Kristiansen

    (BGI-Research
    University of Copenhagen
    BGI-Research)

  • Chen Chen

    (BGI-Research
    University of Copenhagen)

  • Lan Zhu

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

Abstract

Neovaginas are surgically constructed to correct uterovaginal agenesis in women with Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome or as part of gender-affirming surgery for transfeminine individuals. Understanding the assembly of the neovaginal microbiota is crucial for guiding its management. To address this, we conducted a longitudinal study on MRKH patients following laparoscopic peritoneal vaginoplasty. Our findings reveal that the early microbial assemblage exhibited stochastic characteristics, accompanied with a notable bloom of Enterococcus faecalis and genital Mycoplasmas. While both the pre-surgery dimple microbiota and the fecal microbiota constituted the primary species pool, the neovaginal microbiota developed into a microbiota that resembled that of a normal vagina at 6–12 months post-surgery, albeit with a bacterial vaginosis (BV)-like structure. By 2–4 years post-surgery, the neovaginal microbiota had further evolved into a structure closely resembling with the homeostatic pre-surgery dimple microbiota. This concords with the development of the squamous epithelium in the neovagina and highlights the pivotal roles of progressive selective forces imposed by the evolving neovaginal environment and the colonization tropism of vaginal species. Notably, we observed that strains of Lactobacillus crispatus colonizing the neovagina primarily originated from the dimple. Since L. crispatus is generally associated with vaginal health, this finding suggests potential avenues for future research to promote its colonization.

Suggested Citation

  • Na Chen & Lilan Hao & Zhe Zhang & Chenglu Qin & Zhuye Jie & Hongxin Pan & Jiali Duan & Xincheng Huang & Yunhong Zhang & Hongqin Gao & Ruike Lu & Tianshu Sun & Hua Yang & Jinqiu Shi & Maolian Liang & J, 2024. "Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52102-1
    DOI: 10.1038/s41467-024-52102-1
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    References listed on IDEAS

    as
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