IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-52102-1.html
   My bibliography  Save this article

Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients

Author

Listed:
  • Na Chen

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Lilan Hao

    (BGI-Research
    University of Copenhagen
    BGI-Research)

  • Zhe Zhang

    (BGI-Research)

  • Chenglu Qin

    (The 3rd Affiliated Hospital of Shenzhen University, Luohu hospital)

  • Zhuye Jie

    (BGI-Research
    University of Copenhagen)

  • Hongxin Pan

    (Southern University of Science and Technology Hospital)

  • Jiali Duan

    (4+4 Medical Doctor Program, No.9 Dongdansantiao)

  • Xincheng Huang

    (BGI-Research
    BGI-Research)

  • Yunhong Zhang

    (Social Affairs Bureau of Suzhou National New and Hi-tech Industrial Development Zone)

  • Hongqin Gao

    (Suzhou National New and Hi-tech Industrial Development Zone Center for Maternal and Child Health and Family Planning Service)

  • Ruike Lu

    (Suzhou National New and Hi-tech Industrial Development Zone Center for Maternal and Child Health and Family Planning Service)

  • Tianshu Sun

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Hua Yang

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Jinqiu Shi

    (The 3rd Affiliated Hospital of Shenzhen University, Luohu hospital)

  • Maolian Liang

    (The 3rd Affiliated Hospital of Shenzhen University, Luohu hospital)

  • Jianbin Guo

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Qianqian Gao

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Xiaoyue Zhao

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Zhiyuan Dou

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

  • Liang Xiao

    (BGI-Research
    BGI-Research
    BGI-Research)

  • Shaoqiao Zhang

    (BGI Research)

  • Xin Jin

    (BGI-Research)

  • Xun Xu

    (BGI-Research)

  • Huanming Yang

    (BGI-Research
    James D. Watson Institute of Genome Sciences)

  • Jian Wang

    (BGI-Research
    James D. Watson Institute of Genome Sciences)

  • Huijue Jia

    (BGI-Research
    Fudan University
    Fudan University)

  • Tao Zhang

    (BGI Research
    Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research)

  • Karsten Kristiansen

    (BGI-Research
    University of Copenhagen
    BGI-Research)

  • Chen Chen

    (BGI-Research
    University of Copenhagen)

  • Lan Zhu

    (Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan)

Abstract

Neovaginas are surgically constructed to correct uterovaginal agenesis in women with Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome or as part of gender-affirming surgery for transfeminine individuals. Understanding the assembly of the neovaginal microbiota is crucial for guiding its management. To address this, we conducted a longitudinal study on MRKH patients following laparoscopic peritoneal vaginoplasty. Our findings reveal that the early microbial assemblage exhibited stochastic characteristics, accompanied with a notable bloom of Enterococcus faecalis and genital Mycoplasmas. While both the pre-surgery dimple microbiota and the fecal microbiota constituted the primary species pool, the neovaginal microbiota developed into a microbiota that resembled that of a normal vagina at 6–12 months post-surgery, albeit with a bacterial vaginosis (BV)-like structure. By 2–4 years post-surgery, the neovaginal microbiota had further evolved into a structure closely resembling with the homeostatic pre-surgery dimple microbiota. This concords with the development of the squamous epithelium in the neovagina and highlights the pivotal roles of progressive selective forces imposed by the evolving neovaginal environment and the colonization tropism of vaginal species. Notably, we observed that strains of Lactobacillus crispatus colonizing the neovagina primarily originated from the dimple. Since L. crispatus is generally associated with vaginal health, this finding suggests potential avenues for future research to promote its colonization.

Suggested Citation

  • Na Chen & Lilan Hao & Zhe Zhang & Chenglu Qin & Zhuye Jie & Hongxin Pan & Jiali Duan & Xincheng Huang & Yunhong Zhang & Hongqin Gao & Ruike Lu & Tianshu Sun & Hua Yang & Jinqiu Shi & Maolian Liang & J, 2024. "Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52102-1
    DOI: 10.1038/s41467-024-52102-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-52102-1
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-024-52102-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Tao Ding & Patrick D. Schloss, 2014. "Dynamics and associations of microbial community types across the human body," Nature, Nature, vol. 509(7500), pages 357-360, May.
    2. Amir Bashan & Travis E. Gibson & Jonathan Friedman & Vincent J. Carey & Scott T. Weiss & Elizabeth L. Hohmann & Yang-Yu Liu, 2016. "Universality of human microbial dynamics," Nature, Nature, vol. 534(7606), pages 259-262, June.
    3. Chen Chen & Xiaolei Song & Weixia Wei & Huanzi Zhong & Juanjuan Dai & Zhou Lan & Fei Li & Xinlei Yu & Qiang Feng & Zirong Wang & Hailiang Xie & Xiaomin Chen & Chunwei Zeng & Bo Wen & Liping Zeng & Hui, 2017. "The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    4. Yan Shao & Samuel C. Forster & Evdokia Tsaliki & Kevin Vervier & Angela Strang & Nandi Simpson & Nitin Kumar & Mark D. Stares & Alison Rodger & Peter Brocklehurst & Nigel Field & Trevor D. Lawley, 2019. "Stunted microbiota and opportunistic pathogen colonization in caesarean-section birth," Nature, Nature, vol. 574(7776), pages 117-121, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Sean M Gibbons & Sean M Kearney & Chris S Smillie & Eric J Alm, 2017. "Two dynamic regimes in the human gut microbiome," PLOS Computational Biology, Public Library of Science, vol. 13(2), pages 1-20, February.
    2. Joe J. Lim & Christian Diener & James Wilson & Jacob J. Valenzuela & Nitin S. Baliga & Sean M. Gibbons, 2023. "Growth phase estimation for abundant bacterial populations sampled longitudinally from human stool metagenomes," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. He-Li Sun & Yuan Feng & Qinge Zhang & Jia-Xin Li & Yue-Ying Wang & Zhaohui Su & Teris Cheung & Todd Jackson & Sha Sha & Yu-Tao Xiang, 2022. "The Microbiome–Gut–Brain Axis and Dementia: A Bibliometric Analysis," IJERPH, MDPI, vol. 19(24), pages 1-14, December.
    4. Bin Zhu & David J. Edwards & Katherine M. Spaine & Laahirie Edupuganti & Andrey Matveyev & Myrna G. Serrano & Gregory A. Buck, 2024. "The association of maternal factors with the neonatal microbiota and health," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    5. Aneta Słabuszewska-Jóźwiak & Jacek Krzysztof Szymański & Michał Ciebiera & Beata Sarecka-Hujar & Grzegorz Jakiel, 2020. "Pediatrics Consequences of Caesarean Section—A Systematic Review and Meta-Analysis," IJERPH, MDPI, vol. 17(21), pages 1-17, October.
    6. Hunter R Johnson & Donovan D Trinidad & Stephania Guzman & Zenab Khan & James V Parziale & Jennifer M DeBruyn & Nathan H Lents, 2016. "A Machine Learning Approach for Using the Postmortem Skin Microbiome to Estimate the Postmortem Interval," PLOS ONE, Public Library of Science, vol. 11(12), pages 1-23, December.
    7. Krishnagopal, Sanjukta & Bianconi, Ginestra, 2023. "Topology and dynamics of higher-order multiplex networks," Chaos, Solitons & Fractals, Elsevier, vol. 177(C).
    8. Kostroma Ya. V., 2021. "Cost management for various diagnostic schemes for cervicitis and chronic endometritis," Russian Journal of Social Sciences and Humanities, Omsk Humanitarian Academy, vol. 15(3), pages 207-215, September.
    9. Kerstin Thriene & Karin B. Michels, 2023. "Human Gut Microbiota Plasticity throughout the Life Course," IJERPH, MDPI, vol. 20(2), pages 1-14, January.
    10. Lu Wu & Xu-Wen Wang & Zining Tao & Tong Wang & Wenlong Zuo & Yu Zeng & Yang-Yu Liu & Lei Dai, 2024. "Data-driven prediction of colonization outcomes for complex microbial communities," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    11. Zohreh Izadifar & Justin Cotton & Siyu Chen & Viktor Horvath & Anna Stejskalova & Aakanksha Gulati & Nina T. LoGrande & Bogdan Budnik & Sanjid Shahriar & Erin R. Doherty & Yixuan Xie & Tania To & Sara, 2024. "Mucus production, host-microbiome interactions, hormone sensitivity, and innate immune responses modeled in human cervix chips," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    12. Justine Tanoey & Christina Baechle & Hermann Brenner & Andreas Deckert & Julia Fricke & Kathrin Günther & André Karch & Thomas Keil & Alexander Kluttig & Michael Leitzmann & Rafael Mikolajczyk & Nadia, 2022. "Birth Order, Caesarean Section, or Daycare Attendance in Relation to Child- and Adult-Onset Type 1 Diabetes: Results from the German National Cohort," IJERPH, MDPI, vol. 19(17), pages 1-14, August.
    13. C C Lyman & G R Holyoak & K Meinkoth & X Wieneke & K A Chillemi & U DeSilva, 2019. "Canine endometrial and vaginal microbiomes reveal distinct and complex ecosystems," PLOS ONE, Public Library of Science, vol. 14(1), pages 1-17, January.
    14. Brandon Hickman & Anne Salonen & Alise J. Ponsero & Roosa Jokela & Kaija-Leena Kolho & Willem M. Vos & Katri Korpela, 2024. "Gut microbiota wellbeing index predicts overall health in a cohort of 1000 infants," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    15. Maria Nieto-Rosado & Kirsty Sands & Edward A. R. Portal & Kathryn M. Thomson & Maria J. Carvalho & Jordan Mathias & Rebecca Milton & Calie Dyer & Chinenye Akpulu & Ian Boostrom & Patrick Hogan & Habib, 2024. "Colonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    16. Julien Tap & Franck Lejzerowicz & Aurélie Cotillard & Matthieu Pichaud & Daniel McDonald & Se Jin Song & Rob Knight & Patrick Veiga & Muriel Derrien, 2023. "Global branches and local states of the human gut microbiome define associations with environmental and intrinsic factors," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    17. Lena Takayasu & Wataru Suda & Eiichiro Watanabe & Shinji Fukuda & Kageyasu Takanashi & Hiroshi Ohno & Misako Takayasu & Hideki Takayasu & Masahira Hattori, 2017. "A 3-dimensional mathematical model of microbial proliferation that generates the characteristic cumulative relative abundance distributions in gut microbiomes," PLOS ONE, Public Library of Science, vol. 12(8), pages 1-20, August.
    18. Pengfa Li & Leho Tedersoo & Thomas W. Crowther & Baozhan Wang & Yu Shi & Lu Kuang & Ting Li & Meng Wu & Ming Liu & Lu Luan & Jia Liu & Dongzhen Li & Yongxia Li & Songhan Wang & Muhammad Saleem & Alex , 2023. "Global diversity and biogeography of potential phytopathogenic fungi in a changing world," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    19. Bo-Young Hong & Michel V Furtado Araujo & Linda D Strausbaugh & Evimaria Terzi & Effie Ioannidou & Patricia I Diaz, 2015. "Microbiome Profiles in Periodontitis in Relation to Host and Disease Characteristics," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-14, May.
    20. Rajita Menon & Vivek Ramanan & Kirill S Korolev, 2018. "Interactions between species introduce spurious associations in microbiome studies," PLOS Computational Biology, Public Library of Science, vol. 14(1), pages 1-20, January.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52102-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.