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Non-apical mitoses contribute to cell delamination during mouse gastrulation

Author

Listed:
  • Evangéline Despin-Guitard

    (Université Libre de Bruxelles
    UPS)

  • Viviane S. Rosa

    (MRC Laboratory of Molecular Biology)

  • Steffen Plunder

    (UPS
    Sakyo-ku
    Oskar‐Morgenstern‐Platz 1)

  • Navrita Mathiah

    (Université Libre de Bruxelles)

  • Kristof Schoor

    (Université Libre de Bruxelles)

  • Eliana Nehme

    (Université Libre de Bruxelles)

  • Sara Merino-Aceituno

    (Oskar‐Morgenstern‐Platz 1)

  • Joaquim Egea

    (Rovira Roure 80)

  • Marta N. Shahbazi

    (MRC Laboratory of Molecular Biology)

  • Eric Theveneau

    (UPS)

  • Isabelle Migeotte

    (Université Libre de Bruxelles)

Abstract

During the epithelial-mesenchymal transition driving mouse embryo gastrulation, cells divide more frequently at the primitive streak, and half of those divisions happen away from the apical pole. These observations suggest that non-apical mitoses might play a role in cell delamination. We aim to uncover and challenge the molecular determinants of mitosis position in different regions of the epiblast through computational modeling and pharmacological treatments of embryos and stem cell-based epiblast spheroids. Blocking basement membrane degradation at the streak has no impact on the asymmetry in mitosis frequency and position. By contrast, disturbance of the actomyosin cytoskeleton or cell cycle dynamics elicits ectopic non-apical mitosis and shows that the streak region is characterized by local relaxation of the actomyosin cytoskeleton and less stringent regulation of cell division. These factors are essential for normal dynamics at the streak and favor cell delamination from the epiblast.

Suggested Citation

  • Evangéline Despin-Guitard & Viviane S. Rosa & Steffen Plunder & Navrita Mathiah & Kristof Schoor & Eliana Nehme & Sara Merino-Aceituno & Joaquim Egea & Marta N. Shahbazi & Eric Theveneau & Isabelle Mi, 2024. "Non-apical mitoses contribute to cell delamination during mouse gastrulation," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51638-6
    DOI: 10.1038/s41467-024-51638-6
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    References listed on IDEAS

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    1. Christos Kyprianou & Neophytos Christodoulou & Russell S. Hamilton & Wallis Nahaboo & Diana Suarez Boomgaard & Gianluca Amadei & Isabelle Migeotte & Magdalena Zernicka-Goetz, 2020. "Basement membrane remodelling regulates mouse embryogenesis," Nature, Nature, vol. 582(7811), pages 253-258, June.
    2. Christophe Royer & Elizabeth Sandham & Elizabeth Slee & Falk Schneider & Christoffer B. Lagerholm & Jonathan Godwin & Nisha Veits & Holly Hathrell & Felix Zhou & Karolis Leonavicius & Jemma Garratt & , 2022. "ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
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