IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-51021-5.html
   My bibliography  Save this article

Longitudinal proteome-wide antibody profiling in Marburg virus survivors identifies wing domain immunogen for vaccine design

Author

Listed:
  • Surender Khurana

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Gabrielle Grubbs

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Supriya Ravichandran

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Emily Cluff

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • JungHyun Kim

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Ana I. Kuehne

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

  • Samantha Zak

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

  • John M. Dye

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

  • Julius J. Lutwama

    (Emerging, and Re-emerging Infection, Uganda Virus Research Institute)

  • Andrew S. Herbert

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

Abstract

Limited knowledge exists on the quality of polyclonal antibody responses generated following Marburg virus (MARV) infection and its evolution in survivors. In this study, we evaluate MARV proteome-wide antibody repertoire longitudinally in convalescent phase approximately every six months for five years following MARV infection in ten human survivors. Differential kinetics were observed for IgM vs IgG vs IgA epitope diversity, antibody binding, antibody affinity maturation and Fc-receptor interaction to MARV proteins. Durability of MARV-neutralizing antibodies is low in survivors. MARV infection induces a diverse epitope repertoire with predominance against GP, VP40, VP30 and VP24 that persisted up to 5 years post-exposure. However, the IgM and IgA repertoire declines over time. Within MARV-GP, IgG recognize antigenic sites predominantly in the amino-terminus, wing domain and GP2-heptad repeat. Interestingly, MARV infection generates robust durable FcɣRI, FcɣRIIA and FcɣRIIIA IgG-Fc receptor interactions. Immunization with immunodominant MARV epitopes reveals conserved wing region between GP1 and GP2, induces neutralizing antibodies against MARV. These findings demonstrate that MARV infection generates a diverse, long-lasting, non-neutralizing, IgG antibody repertoire that perturbs disease by FcɣR activity. This information, along with discovery of neutralizing immunogen in wing domain, could aid in development of effective therapeutics and vaccines against Marburg virus.

Suggested Citation

  • Surender Khurana & Gabrielle Grubbs & Supriya Ravichandran & Emily Cluff & JungHyun Kim & Ana I. Kuehne & Samantha Zak & John M. Dye & Julius J. Lutwama & Andrew S. Herbert, 2024. "Longitudinal proteome-wide antibody profiling in Marburg virus survivors identifies wing domain immunogen for vaccine design," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51021-5
    DOI: 10.1038/s41467-024-51021-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-51021-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-51021-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Surender Khurana & Megan Hahn & Elizabeth M. Coyle & Lisa R. King & Tsai-Lien Lin & John Treanor & Andrea Sant & Hana Golding, 2019. "Repeat vaccination reduces antibody affinity maturation across different influenza vaccine platforms in humans," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    2. Xiangguo Qiu & Gary Wong & Jonathan Audet & Alexander Bello & Lisa Fernando & Judie B. Alimonti & Hugues Fausther-Bovendo & Haiyan Wei & Jenna Aviles & Ernie Hiatt & Ashley Johnson & Josh Morton & Kel, 2014. "Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp," Nature, Nature, vol. 514(7520), pages 47-53, October.
    3. Robert W. Cross & Zachary A. Bornholdt & Abhishek N. Prasad & Viktoriya Borisevich & Krystle N. Agans & Daniel J. Deer & Dafna M. Abelson & Do H. Kim & William S. Shestowsky & Lioudmila A. Campbell & , 2021. "Combination therapy protects macaques against advanced Marburg virus disease," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    4. Ewen Callaway, 2023. "Marburg virus outbreak: researchers race to test vaccines," Nature, Nature, vol. 614(7949), pages 603-603, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Wataru Nishi & Ei Wakamatsu & Hiroaki Machiyama & Ryohei Matsushima & Kensho Saito & Yosuke Yoshida & Tetsushi Nishikawa & Tomohiro Takehara & Hiroko Toyota & Masae Furuhata & Hitoshi Nishijima & Arat, 2023. "Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Franziska K. Kaiser & Mariana Gonzalez Hernandez & Nadine Krüger & Ellinor Englund & Wenjuan Du & Anna Z. Mykytyn & Mathijs P. Raadsen & Mart M. Lamers & Francine Rodrigues Ianiski & Tatiana M. Shamor, 2024. "Filamentous fungus-produced human monoclonal antibody provides protection against SARS-CoV-2 in hamster and non-human primate models," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Feng Liu & F. Liaini Gross & Sneha Joshi & Manjusha Gaglani & Allison L. Naleway & Kempapura Murthy & Holly C. Groom & Meredith G. Wesley & Laura J. Edwards & Lauren Grant & Sara S. Kim & Suryaprakash, 2024. "Redirecting antibody responses from egg-adapted epitopes following repeat vaccination with recombinant or cell culture-based versus egg-based influenza vaccines," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Ji Wang & Caiguangxi Deng & Ming Liu & Yihao Liu & Liubing Li & Zhangping Huang & Liru Shang & Juan Jiang & Yongyong Li & Ruohui Mo & Hui Zhang & Min Liu & Sui Peng & Haipeng Xiao, 2022. "A fourth dose of the inactivated SARS-CoV-2 vaccine redistributes humoral immunity to the N-terminal domain," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    5. Zhang, Kebo & Hong, Xiao & Han, Yuexing & Wang, Bing, 2023. "Optimal discrete resource allocation on metapopulation networks for suppressing spatial spread of epidemic," Chaos, Solitons & Fractals, Elsevier, vol. 169(C).

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51021-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.