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Longitudinal proteome-wide antibody profiling in Marburg virus survivors identifies wing domain immunogen for vaccine design

Author

Listed:
  • Surender Khurana

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Gabrielle Grubbs

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Supriya Ravichandran

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Emily Cluff

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • JungHyun Kim

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Ana I. Kuehne

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

  • Samantha Zak

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

  • John M. Dye

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

  • Julius J. Lutwama

    (Emerging, and Re-emerging Infection, Uganda Virus Research Institute)

  • Andrew S. Herbert

    (U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick)

Abstract

Limited knowledge exists on the quality of polyclonal antibody responses generated following Marburg virus (MARV) infection and its evolution in survivors. In this study, we evaluate MARV proteome-wide antibody repertoire longitudinally in convalescent phase approximately every six months for five years following MARV infection in ten human survivors. Differential kinetics were observed for IgM vs IgG vs IgA epitope diversity, antibody binding, antibody affinity maturation and Fc-receptor interaction to MARV proteins. Durability of MARV-neutralizing antibodies is low in survivors. MARV infection induces a diverse epitope repertoire with predominance against GP, VP40, VP30 and VP24 that persisted up to 5 years post-exposure. However, the IgM and IgA repertoire declines over time. Within MARV-GP, IgG recognize antigenic sites predominantly in the amino-terminus, wing domain and GP2-heptad repeat. Interestingly, MARV infection generates robust durable FcɣRI, FcɣRIIA and FcɣRIIIA IgG-Fc receptor interactions. Immunization with immunodominant MARV epitopes reveals conserved wing region between GP1 and GP2, induces neutralizing antibodies against MARV. These findings demonstrate that MARV infection generates a diverse, long-lasting, non-neutralizing, IgG antibody repertoire that perturbs disease by FcɣR activity. This information, along with discovery of neutralizing immunogen in wing domain, could aid in development of effective therapeutics and vaccines against Marburg virus.

Suggested Citation

  • Surender Khurana & Gabrielle Grubbs & Supriya Ravichandran & Emily Cluff & JungHyun Kim & Ana I. Kuehne & Samantha Zak & John M. Dye & Julius J. Lutwama & Andrew S. Herbert, 2024. "Longitudinal proteome-wide antibody profiling in Marburg virus survivors identifies wing domain immunogen for vaccine design," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51021-5
    DOI: 10.1038/s41467-024-51021-5
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    References listed on IDEAS

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    1. Xiangguo Qiu & Gary Wong & Jonathan Audet & Alexander Bello & Lisa Fernando & Judie B. Alimonti & Hugues Fausther-Bovendo & Haiyan Wei & Jenna Aviles & Ernie Hiatt & Ashley Johnson & Josh Morton & Kel, 2014. "Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp," Nature, Nature, vol. 514(7520), pages 47-53, October.
    2. Robert W. Cross & Zachary A. Bornholdt & Abhishek N. Prasad & Viktoriya Borisevich & Krystle N. Agans & Daniel J. Deer & Dafna M. Abelson & Do H. Kim & William S. Shestowsky & Lioudmila A. Campbell & , 2021. "Combination therapy protects macaques against advanced Marburg virus disease," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    3. Surender Khurana & Megan Hahn & Elizabeth M. Coyle & Lisa R. King & Tsai-Lien Lin & John Treanor & Andrea Sant & Hana Golding, 2019. "Repeat vaccination reduces antibody affinity maturation across different influenza vaccine platforms in humans," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    4. Ewen Callaway, 2023. "Marburg virus outbreak: researchers race to test vaccines," Nature, Nature, vol. 614(7949), pages 603-603, February.
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