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Uridylation regulates mRNA decay directionality in fission yeast

Author

Listed:
  • Maciej Grochowski

    (University of Warsaw)

  • Lidia Lipińska-Zubrycka

    (University of Warsaw)

  • StJohn Townsend

    (Charité - Universitätsmedizin Berlin
    Francis Crick Institute)

  • Anna Golisz-Mocydlarz

    (University of Warsaw)

  • Monika Zakrzewska-Płaczek

    (University of Warsaw)

  • Grzegorz Brzyżek

    (PAS)

  • Borna Jurković

    (University of Warsaw)

  • Szymon Świeżewski

    (PAS)

  • Markus Ralser

    (Charité - Universitätsmedizin Berlin
    Francis Crick Institute)

  • Michał Małecki

    (University of Warsaw)

Abstract

Cytoplasmic mRNA decay is effected by exonucleolytic degradation in either the 5’ to 3’ or 3’ to 5’ direction. Pervasive terminal uridylation is implicated in mRNA degradation, however, its functional relevance for bulk mRNA turnover remains poorly understood. In this study, we employ genome-wide 3’-RACE (gw3’-RACE) in the model system fission yeast to elucidate the role of uridylation in mRNA turnover. We observe widespread uridylation of shortened poly(A) tails, promoting efficient 5’ to 3’ mRNA decay and ensuring timely and controlled mRNA degradation. Inhibition of this uridylation process leads to excessive deadenylation and enhanced 3’ to 5’ mRNA decay accompanied by oligouridylation. Strikingly we found that uridylation of poly(A) tails and oligouridylation of non-polyadenylated substrates are catalysed by different terminal uridyltransferases Cid1 and Cid16 respectively. Our study sheds new light on the intricate regulatory mechanisms underlying bulk mRNA turnover, demonstrating the role of uridylation in modulating mRNA decay pathways.

Suggested Citation

  • Maciej Grochowski & Lidia Lipińska-Zubrycka & StJohn Townsend & Anna Golisz-Mocydlarz & Monika Zakrzewska-Płaczek & Grzegorz Brzyżek & Borna Jurković & Szymon Świeżewski & Markus Ralser & Michał Małec, 2024. "Uridylation regulates mRNA decay directionality in fission yeast," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50824-w
    DOI: 10.1038/s41467-024-50824-w
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    References listed on IDEAS

    as
    1. Agnieszka Tudek & Paweł S. Krawczyk & Seweryn Mroczek & Rafał Tomecki & Matti Turtola & Katarzyna Matylla-Kulińska & Torben Heick Jensen & Andrzej Dziembowski, 2021. "Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Paola Pisacane & Mario Halic, 2017. "Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi," Nature Communications, Nature, vol. 8(1), pages 1-13, August.
    3. Hélène Scheer & Caroline Almeida & Emilie Ferrier & Quentin Simonnot & Laure Poirier & David Pflieger & François M. Sement & Sandrine Koechler & Christina Piermaria & Paweł Krawczyk & Seweryn Mroczek , 2021. "The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
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