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Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae

Author

Listed:
  • Agnieszka Tudek

    (Institute of Biochemistry and Biophysics)

  • Paweł S. Krawczyk

    (Institute of Biochemistry and Biophysics
    International Institute of Molecular and Cell Biology)

  • Seweryn Mroczek

    (International Institute of Molecular and Cell Biology
    University of Warsaw)

  • Rafał Tomecki

    (Institute of Biochemistry and Biophysics
    University of Warsaw)

  • Matti Turtola

    (Aarhus University)

  • Katarzyna Matylla-Kulińska

    (International Institute of Molecular and Cell Biology
    University of Warsaw)

  • Torben Heick Jensen

    (Aarhus University)

  • Andrzej Dziembowski

    (Institute of Biochemistry and Biophysics
    International Institute of Molecular and Cell Biology
    University of Warsaw)

Abstract

The polyadenosine tail (poly[A]-tail) is a universal modification of eukaryotic messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). In budding yeast, Pap1-synthesized mRNA poly(A) tails enhance export and translation, whereas Trf4/5-mediated polyadenylation of ncRNAs facilitates degradation by the exosome. Using direct RNA sequencing, we decipher the extent of poly(A) tail dynamics in yeast defective in all relevant exonucleases, deadenylases, and poly(A) polymerases. Predominantly ncRNA poly(A) tails are 20-60 adenosines long. Poly(A) tails of newly transcribed mRNAs are 50 adenosine long on average, with an upper limit of 200. Exonucleolysis by Trf5-assisted nuclear exosome and cytoplasmic deadenylases trim the tails to 40 adenosines on average. Surprisingly, PAN2/3 and CCR4-NOT deadenylase complexes have a large pool of non-overlapping substrates mainly defined by expression level. Finally, we demonstrate that mRNA poly(A) tail length strongly responds to growth conditions, such as heat and nutrient deprivation.

Suggested Citation

  • Agnieszka Tudek & Paweł S. Krawczyk & Seweryn Mroczek & Rafał Tomecki & Matti Turtola & Katarzyna Matylla-Kulińska & Torben Heick Jensen & Andrzej Dziembowski, 2021. "Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25251-w
    DOI: 10.1038/s41467-021-25251-w
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    Cited by:

    1. Maciej Grochowski & Lidia Lipińska-Zubrycka & StJohn Townsend & Anna Golisz-Mocydlarz & Monika Zakrzewska-Płaczek & Grzegorz Brzyżek & Borna Jurković & Szymon Świeżewski & Markus Ralser & Michał Małec, 2024. "Uridylation regulates mRNA decay directionality in fission yeast," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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