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Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi

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  • Paola Pisacane

    (Gene Center, University of Munich)

  • Mario Halic

    (Gene Center, University of Munich)

Abstract

RNAi is a conserved mechanism in which small RNAs induce silencing of complementary targets. How Argonaute-bound small RNAs are targeted for degradation is not well understood. We show that the adenyl-transferase Cid14, a member of the TRAMP complex, and the uridyl-transferase Cid16 add non-templated nucleotides to Argonaute-bound small RNAs in fission yeast. The tailing of Argonaute-bound small RNAs recruits the 3′–5′ exonuclease Rrp6 to degrade small RNAs. Failure in degradation of Argonaute-bound small RNAs results in accumulation of ‘noise’ small RNAs on Argonaute and targeting of diverse euchromatic genes by RNAi. To protect themselves from uncontrolled RNAi, cid14Δ cells exploit the RNAi machinery and silence genes essential for RNAi itself, which is required for their viability. Our data indicate that surveillance of Argonaute-bound small RNAs by Cid14/Cid16 and the exosome protects the genome from uncontrolled RNAi and reveal a rapid RNAi-based adaptation to stress conditions.

Suggested Citation

  • Paola Pisacane & Mario Halic, 2017. "Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi," Nature Communications, Nature, vol. 8(1), pages 1-13, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15332
    DOI: 10.1038/ncomms15332
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    Cited by:

    1. Maciej Grochowski & Lidia Lipińska-Zubrycka & StJohn Townsend & Anna Golisz-Mocydlarz & Monika Zakrzewska-Płaczek & Grzegorz Brzyżek & Borna Jurković & Szymon Świeżewski & Markus Ralser & Michał Małec, 2024. "Uridylation regulates mRNA decay directionality in fission yeast," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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