IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-49695-y.html
   My bibliography  Save this article

Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes

Author

Listed:
  • Yusheng Liu

    (Northeast Forestry University
    Chinese Academy of Sciences)

  • Wenrong Tao

    (Shandong University)

  • Shuang Wu

    (Chinese Academy of Sciences
    Northeast Agricultural University)

  • Yiwei Zhang

    (Chinese Academy of Sciences
    Northeast Agricultural University)

  • Hu Nie

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhenzhen Hou

    (Shandong University)

  • Jingye Zhang

    (Shandong University)

  • Zhen Yang

    (Shandong University)

  • Zi-Jiang Chen

    (Shandong University
    Chinese Academy of Medical Sciences (No. 2021RU001))

  • Jiaqiang Wang

    (Northeast Agricultural University)

  • Falong Lu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Keliang Wu

    (Shandong University)

Abstract

Oocyte in vitro maturation is a technique in assisted reproductive technology. Thousands of genes show abnormally high expression in in vitro maturated metaphase II (MII) oocytes compared to those matured in vivo in bovines, mice, and humans. The mechanisms underlying this phenomenon are poorly understood. Here, we use poly(A) inclusive RNA isoform sequencing (PAIso-seq) for profiling the transcriptome-wide poly(A) tails in both in vivo and in vitro matured mouse and human oocytes. Our results demonstrate that the observed increase in maternal mRNA abundance is caused by impaired deadenylation in in vitro MII oocytes. Moreover, the cytoplasmic polyadenylation of dormant Btg4 and Cnot7 mRNAs, which encode key components of deadenylation machinery, is impaired in in vitro MII oocytes, contributing to reduced translation of these deadenylase machinery components and subsequently impaired global maternal mRNA deadenylation. Our findings highlight impaired maternal mRNA deadenylation as a distinct molecular defect in in vitro MII oocytes.

Suggested Citation

  • Yusheng Liu & Wenrong Tao & Shuang Wu & Yiwei Zhang & Hu Nie & Zhenzhen Hou & Jingye Zhang & Zhen Yang & Zi-Jiang Chen & Jiaqiang Wang & Falong Lu & Keliang Wu, 2024. "Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49695-y
    DOI: 10.1038/s41467-024-49695-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-49695-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-49695-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Yusheng Liu & Hu Nie & Hongxiang Liu & Falong Lu, 2019. "Poly(A) inclusive RNA isoform sequencing (PAIso−seq) reveals wide-spread non-adenosine residues within RNA poly(A) tails," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    2. Nobuhiko Hamazaki & Hirohisa Kyogoku & Hiromitsu Araki & Fumihito Miura & Chisako Horikawa & Norio Hamada & So Shimamoto & Orie Hikabe & Kinichi Nakashima & Tomoya S. Kitajima & Takashi Ito & Harry G., 2021. "Reconstitution of the oocyte transcriptional network with transcription factors," Nature, Nature, vol. 589(7841), pages 264-269, January.
    3. Orie Hikabe & Nobuhiko Hamazaki & Go Nagamatsu & Yayoi Obata & Yuji Hirao & Norio Hamada & So Shimamoto & Takuya Imamura & Kinichi Nakashima & Mitinori Saitou & Katsuhiko Hayashi, 2016. "Reconstitution in vitro of the entire cycle of the mouse female germ line," Nature, Nature, vol. 539(7628), pages 299-303, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ilmatar Rooda & Jasmin Hassan & Jie Hao & Magdalena Wagner & Elisabeth Moussaud-Lamodière & Kersti Jääger & Marjut Otala & Katri Knuus & Cecilia Lindskog & Kiriaki Papaikonomou & Sebastian Gidlöf & Ce, 2024. "In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Ryuki Shimada & Yuzuru Kato & Naoki Takeda & Sayoko Fujimura & Kei-ichiro Yasunaga & Shingo Usuki & Hitoshi Niwa & Kimi Araki & Kei-ichiro Ishiguro, 2023. "STRA8–RB interaction is required for timely entry of meiosis in mouse female germ cells," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Mengwen Hu & Yu-Han Yeh & Yasuhisa Munakata & Hironori Abe & Akihiko Sakashita & So Maezawa & Miguel Vidal & Haruhiko Koseki & Neil Hunter & Richard M. Schultz & Satoshi H. Namekawa, 2022. "PRC1-mediated epigenetic programming is required to generate the ovarian reserve," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49695-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.