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High-speed optical imaging with sCMOS pixel reassignment

Author

Listed:
  • Biagio Mandracchia

    (Georgia Institute of Technology and Emory University
    Universidad de Valladolid)

  • Corey Zheng

    (Georgia Institute of Technology and Emory University)

  • Suraj Rajendran

    (Georgia Institute of Technology and Emory University)

  • Wenhao Liu

    (Georgia Institute of Technology and Emory University)

  • Parvin Forghani

    (Emory University)

  • Chunhui Xu

    (Emory University
    Georgia Institute of Technology)

  • Shu Jia

    (Georgia Institute of Technology and Emory University
    Georgia Institute of Technology)

Abstract

Fluorescence microscopy has undergone rapid advancements, offering unprecedented visualization of biological events and shedding light on the intricate mechanisms governing living organisms. However, the exploration of rapid biological dynamics still poses a significant challenge due to the limitations of current digital camera architectures and the inherent compromise between imaging speed and other capabilities. Here, we introduce sHAPR, a high-speed acquisition technique that leverages the operating principles of sCMOS cameras to capture fast cellular and subcellular processes. sHAPR harnesses custom fiber optics to convert microscopy images into one-dimensional recordings, enabling acquisition at the maximum camera readout rate, typically between 25 and 250 kHz. We have demonstrated the utility of sHAPR with a variety of phantom and dynamic systems, including high-throughput flow cytometry, cardiomyocyte contraction, and neuronal calcium waves, using a standard epi-fluorescence microscope. sHAPR is highly adaptable and can be integrated into existing microscopy systems without requiring extensive platform modifications. This method pushes the boundaries of current fluorescence imaging capabilities, opening up new avenues for investigating high-speed biological phenomena.

Suggested Citation

  • Biagio Mandracchia & Corey Zheng & Suraj Rajendran & Wenhao Liu & Parvin Forghani & Chunhui Xu & Shu Jia, 2024. "High-speed optical imaging with sCMOS pixel reassignment," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48987-7
    DOI: 10.1038/s41467-024-48987-7
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    References listed on IDEAS

    as
    1. Yonatan Israel & Ron Tenne & Dan Oron & Yaron Silberberg, 2017. "Quantum correlation enhanced super-resolution localization microscopy enabled by a fibre bundle camera," Nature Communications, Nature, vol. 8(1), pages 1-5, April.
    2. Mototaka Suzuki & Matthew E. Larkum, 2017. "Dendritic calcium spikes are clearly detectable at the cortical surface," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    3. Liang Gao & Jinyang Liang & Chiye Li & Lihong V. Wang, 2014. "Single-shot compressed ultrafast photography at one hundred billion frames per second," Nature, Nature, vol. 516(7529), pages 74-77, December.
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