IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-48140-4.html
   My bibliography  Save this article

The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum

Author

Listed:
  • Hui Min

    (University of South Florida
    China Medical University)

  • Xiaoying Liang

    (University of South Florida)

  • Chengqi Wang

    (University of South Florida)

  • Junling Qin

    (University of South Florida)

  • Rachasak Boonhok

    (University of South Florida
    Walailak University)

  • Azhar Muneer

    (University of South Florida)

  • Awtum M. Brashear

    (University of South Florida)

  • Xiaolian Li

    (University of South Florida)

  • Allen M. Minns

    (Pennsylvania State University)

  • Swamy Rakesh Adapa

    (University of South Florida)

  • Rays H. Y. Jiang

    (University of South Florida)

  • Gang Ning

    (Pennsylvania State University)

  • Yaming Cao

    (China Medical University)

  • Scott E. Lindner

    (Pennsylvania State University)

  • Jun Miao

    (University of South Florida)

  • Liwang Cui

    (University of South Florida)

Abstract

In malaria parasites, the regulation of mRNA translation, storage and degradation during development and life-stage transitions remains largely unknown. Here, we functionally characterized the DEAD-box RNA helicase PfDOZI in P. falciparum. Disruption of pfdozi enhanced asexual proliferation but reduced sexual commitment and impaired gametocyte development. By quantitative transcriptomics, we show that PfDOZI is involved in the regulation of invasion-related genes and sexual stage-specific genes during different developmental stages. PfDOZI predominantly participates in processing body-like mRNPs in schizonts but germ cell granule-like mRNPs in gametocytes to impose opposing actions of degradation and protection on different mRNA targets. We further show the formation of stress granule-like mRNPs during nutritional deprivation, highlighting an essential role of PfDOZI-associated mRNPs in stress response. We demonstrate that PfDOZI participates in distinct mRNPs to maintain mRNA homeostasis in response to life-stage transition and environmental changes by differentially executing post-transcriptional regulation on the target mRNAs.

Suggested Citation

  • Hui Min & Xiaoying Liang & Chengqi Wang & Junling Qin & Rachasak Boonhok & Azhar Muneer & Awtum M. Brashear & Xiaolian Li & Allen M. Minns & Swamy Rakesh Adapa & Rays H. Y. Jiang & Gang Ning & Yaming , 2024. "The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48140-4
    DOI: 10.1038/s41467-024-48140-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-48140-4
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-024-48140-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Suresh M. Ganesan & Alejandra Falla & Stephen J. Goldfless & Armiyaw S. Nasamu & Jacquin C. Niles, 2016. "Synthetic RNA–protein modules integrated with native translation mechanisms to control gene expression in malaria parasites," Nature Communications, Nature, vol. 7(1), pages 1-10, April.
    2. Min Zhang & Chengqi Wang & Jenna Oberstaller & Phaedra Thomas & Thomas D. Otto & Debora Casandra & Sandhya Boyapalle & Swamy R. Adapa & Shulin Xu & Katrina Button-Simons & Matthew Mayho & Julian C. Ra, 2021. "The apicoplast link to fever-survival and artemisinin-resistance in the malaria parasite," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    3. Jessica L. Bridgford & Stanley C. Xie & Simon A. Cobbold & Charisse Flerida A. Pasaje & Susann Herrmann & Tuo Yang & David L. Gillett & Lawrence R. Dick & Stuart A. Ralph & Con Dogovski & Natalie J. S, 2018. "Artemisinin kills malaria parasites by damaging proteins and inhibiting the proteasome," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Stanley C. Xie & Yinuo Wang & Craig J. Morton & Riley D. Metcalfe & Con Dogovski & Charisse Flerida A. Pasaje & Elyse Dunn & Madeline R. Luth & Krittikorn Kumpornsin & Eva S. Istvan & Joon Sung Park &, 2024. "Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNA synthetase," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Deyun Qiu & Jinxin V. Pei & James E. O. Rosling & Vandana Thathy & Dongdi Li & Yi Xue & John D. Tanner & Jocelyn Sietsma Penington & Yi Tong Vincent Aw & Jessica Yi Han Aw & Guoyue Xu & Abhai K. Tripa, 2022. "A G358S mutation in the Plasmodium falciparum Na+ pump PfATP4 confers clinically-relevant resistance to cipargamin," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    3. Marvin Chew & Weijian Ye & Radoslaw Igor Omelianczyk & Charisse Flerida Pasaje & Regina Hoo & Qingfeng Chen & Jacquin C. Niles & Jianzhu Chen & Peter Preiser, 2022. "Selective expression of variant surface antigens enables Plasmodium falciparum to evade immune clearance in vivo," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Laura E. Vries & Patrick A. M. Jansen & Catalina Barcelo & Justin Munro & Julie M. J. Verhoef & Charisse Flerida A. Pasaje & Kelly Rubiano & Josefine Striepen & Nada Abla & Luuk Berning & Judith M. Bo, 2022. "Preclinical characterization and target validation of the antimalarial pantothenamide MMV693183," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    5. Thomas Hollin & Steven Abel & Alejandra Falla & Charisse Flerida A. Pasaje & Anil Bhatia & Manhoi Hur & Jay S. Kirkwood & Anita Saraf & Jacques Prudhomme & Amancio De Souza & Laurence Florens & Jacqui, 2022. "Functional genomics of RAP proteins and their role in mitoribosome regulation in Plasmodium falciparum," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    6. Alexander A. Morano & Rachel M. Rudlaff & Jeffrey D. Dvorin, 2023. "A PPP-type pseudophosphatase is required for the maintenance of basal complex integrity in Plasmodium falciparum," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48140-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.