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A widely conserved protein Rof inhibits transcription termination factor Rho and promotes Salmonella virulence program

Author

Listed:
  • Jing Zhang

    (Chinese Academy of Sciences)

  • Shuo Zhang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Wei Zhou

    (Beijing Institute of Radiation Medicine)

  • Xiang Zhang

    (Fudan University)

  • Guanjin Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Ruoxuan Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xingyu Lin

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Ziying Chen

    (Chinese Academy of Sciences
    Fudan University)

  • Fang Liu

    (Chinese Academy of Sciences)

  • Pan Shen

    (Beijing Institute of Radiation Medicine)

  • Xiaogen Zhou

    (Zhejiang University of Technology)

  • Yue Gao

    (Beijing Institute of Radiation Medicine)

  • Zhenguo Chen

    (Fudan University)

  • Yanjie Chao

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Chengyuan Wang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Transcription is crucial for the expression of genetic information and its efficient and accurate termination is required for all living organisms. Rho-dependent termination could rapidly terminate unwanted premature RNAs and play important roles in bacterial adaptation to changing environments. Although Rho has been discovered for about five decades, the regulation mechanisms of Rho-dependent termination are still not fully elucidated. Here we report that Rof is a conserved antiterminator and determine the cryogenic electron microscopy structure of Rho-Rof antitermination complex. Rof binds to the open-ring Rho hexamer and inhibits the initiation of Rho-dependent termination. Rof’s N-terminal α-helix undergoes conformational changes upon binding with Rho, and is key in facilitating Rof-Rho interactions. Rof binds to Rho’s primary binding site (PBS) and excludes Rho from binding with PBS ligand RNA at the initiation step. Further in vivo analyses in Salmonella Typhimurium show that Rof is required for virulence gene expression and host cell invasion, unveiling a physiological function of Rof and transcription termination in bacterial pathogenesis.

Suggested Citation

  • Jing Zhang & Shuo Zhang & Wei Zhou & Xiang Zhang & Guanjin Li & Ruoxuan Li & Xingyu Lin & Ziying Chen & Fang Liu & Pan Shen & Xiaogen Zhou & Yue Gao & Zhenguo Chen & Yanjie Chao & Chengyuan Wang, 2024. "A widely conserved protein Rof inhibits transcription termination factor Rho and promotes Salmonella virulence program," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47438-7
    DOI: 10.1038/s41467-024-47438-7
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    References listed on IDEAS

    as
    1. Vitaly Epshtein & Dipak Dutta & Joseph Wade & Evgeny Nudler, 2010. "An allosteric mechanism of Rho-dependent transcription termination," Nature, Nature, vol. 463(7278), pages 245-249, January.
    2. Vadim Molodtsov & Chengyuan Wang & Emre Firlar & Jason T. Kaelber & Richard H. Ebright, 2023. "Structural basis of Rho-dependent transcription termination," Nature, Nature, vol. 614(7947), pages 367-374, February.
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