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Structural basis of Rho-dependent transcription termination

Author

Listed:
  • Vadim Molodtsov

    (Rutgers University)

  • Chengyuan Wang

    (Rutgers University)

  • Emre Firlar

    (Rutgers University)

  • Jason T. Kaelber

    (Rutgers University)

  • Richard H. Ebright

    (Rutgers University)

Abstract

Rho is a ring-shaped hexameric ATP-dependent molecular motor. Together with the transcription elongation factor NusG, Rho mediates factor-dependent transcription termination and transcription–translation-coupling quality control in Escherichia coli1–4. Here we report the preparation of complexes that are functional in factor-dependent transcription termination from Rho, NusG, RNA polymerase (RNAP), and synthetic nucleic acid scaffolds, and we report cryogenic electron microscopy structures of the complexes. The structures show that functional factor-dependent pre-termination complexes contain a closed-ring Rho hexamer; have RNA threaded through the central channel of Rho; have 60 nucleotides of RNA interacting sequence-specifically with the exterior of Rho and 6 nucleotides of RNA interacting sequence-specifically with the central channel of Rho; have Rho oriented relative to RNAP such that ATP-dependent translocation by Rho exerts mechanical force on RNAP; and have NusG bridging Rho and RNAP. The results explain five decades of research on Rho and provide a foundation for understanding Rho’s function.

Suggested Citation

  • Vadim Molodtsov & Chengyuan Wang & Emre Firlar & Jason T. Kaelber & Richard H. Ebright, 2023. "Structural basis of Rho-dependent transcription termination," Nature, Nature, vol. 614(7947), pages 367-374, February.
    • Handle: RePEc:nat:nature:v:614:y:2023:i:7947:d:10.1038_s41586-022-05658-1
    DOI: 10.1038/s41586-022-05658-1
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    Cited by:

    1. Jing Zhang & Shuo Zhang & Wei Zhou & Xiang Zhang & Guanjin Li & Ruoxuan Li & Xingyu Lin & Ziying Chen & Fang Liu & Pan Shen & Xiaogen Zhou & Yue Gao & Zhenguo Chen & Yanjie Chao & Chengyuan Wang, 2024. "A widely conserved protein Rof inhibits transcription termination factor Rho and promotes Salmonella virulence program," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Emily Petroni & Caroline Esnault & Daniel Tetreault & Ryan K. Dale & Gisela Storz & Philip P. Adams, 2023. "Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen Borrelia burgdorferi," Nature Communications, Nature, vol. 14(1), pages 1-23, December.
    3. Tatsuo Yanagisawa & Yuko Murayama & Haruhiko Ehara & Mie Goto & Mari Aoki & Shun-ichi Sekine, 2024. "Structural basis of eukaryotic transcription termination by the Rat1 exonuclease complex," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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