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Nanopore analysis of cis-diols in fruits

Author

Listed:
  • Pingping Fan

    (Nanjing University
    Nanjing University)

  • Zhenyuan Cao

    (Nanjing University
    Nanjing University)

  • Shanyu Zhang

    (Nanjing University
    Nanjing University)

  • Yuqin Wang

    (Nanjing University
    Nanjing University
    Nanjing University
    Nanjing University Suzhou Campus)

  • Yunqi Xiao

    (Nanjing University
    Nanjing University)

  • Wendong Jia

    (Nanjing University
    Nanjing University)

  • Panke Zhang

    (Nanjing University)

  • Shuo Huang

    (Nanjing University
    Nanjing University)

Abstract

Natural fruits contain a large variety of cis-diols. However, due to the lack of a high-resolution sensor that can simultaneously identify all cis-diols without a need of complex sample pretreatment, direct and rapid analysis of fruits in a hand-held device has never been previously reported. Nanopore, a versatile single molecule sensor, can be specially engineered to perform this task. A hetero-octameric Mycobacterium smegmatis porin A (MspA) nanopore modified with a sole phenylboronic acid (PBA) adapter is prepared. This engineered MspA accurately recognizes 1,2-diphenols, alditols, α-hydroxy acids and saccharides in prune, grape, lemon, different varieties of kiwifruits and commercial juice products. Assisted with a custom machine learning program, an accuracy of 99.3% is reported and the sample pretreatment is significantly simplified. Enantiomers such as DL-malic acids can also be directly identified, enabling sensing of synthetic food additives. Though demonstrated with fruits, these results suggest wide applications of nanopore in food and drug administration uses.

Suggested Citation

  • Pingping Fan & Zhenyuan Cao & Shanyu Zhang & Yuqin Wang & Yunqi Xiao & Wendong Jia & Panke Zhang & Shuo Huang, 2024. "Nanopore analysis of cis-diols in fruits," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46303-x
    DOI: 10.1038/s41467-024-46303-x
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    References listed on IDEAS

    as
    1. Wendong Jia & Chengzhen Hu & Yuqin Wang & Yuming Gu & Guangrui Qian & Xiaoyu Du & Liying Wang & Yao Liu & Jiao Cao & Shanyu Zhang & Shuanghong Yan & Panke Zhang & Jing Ma & Hong-Yuan Chen & Shuo Huang, 2021. "Programmable nano-reactors for stochastic sensing," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    2. Yuqin Wang & Xiaoyu Guan & Shanyu Zhang & Yao Liu & Sha Wang & Pingping Fan & Xiaoyu Du & Shuanghong Yan & Panke Zhang & Hong-Yuan Chen & Wenfei Li & Daoqiang Zhang & Shuo Huang, 2021. "Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    3. Jiao Cao & Wendong Jia & Jinyue Zhang & Xiumei Xu & Shuanghong Yan & Yuqin Wang & Panke Zhang & Hong-Yuan Chen & Shuo Huang, 2019. "Giant single molecule chemistry events observed from a tetrachloroaurate(III) embedded Mycobacterium smegmatis porin A nanopore," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    4. Fabien Piguet & Hadjer Ouldali & Manuela Pastoriza-Gallego & Philippe Manivet & Juan Pelta & Abdelghani Oukhaled, 2018. "Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
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