Author
Listed:
- Jiao Cao
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University)
- Wendong Jia
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University)
- Jinyue Zhang
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University)
- Xiumei Xu
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University
Nanyang Normal University)
- Shuanghong Yan
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University)
- Yuqin Wang
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University)
- Panke Zhang
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University)
- Hong-Yuan Chen
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University)
- Shuo Huang
(State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University
Nanjing University
Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University)
Abstract
Biological nanopores are capable of resolving small analytes down to a monoatomic ion. In this research, tetrachloroaurate(III), a polyatomic ion, is discovered to bind to the methionine residue (M113) of a wild-type α-hemolysin by reversible Au(III)-thioether coordination. However, the cylindrical pore geometry of α-hemolysin generates shallow ionic binding events (~5–6 pA) and may have introduced other undesired interactions. Inspired by nanopore sequencing, a Mycobacterium smegmatis porin A (MspA) nanopore, which possesses a conical pore geometry, is mutated to bind tetrachloroaurate(III). Subsequently, further amplified blockage events (up to ~55 pA) are observed, which report the largest single ion binding event from a nanopore measurement. By taking the embedded Au(III) as an atomic bridge, the MspA nanopore is enabled to discriminate between different biothiols from single molecule readouts. These phenomena suggest that MspA is advantageous for single molecule chemistry investigations and has applications as a hybrid biological nanopore with atomic adaptors.
Suggested Citation
Jiao Cao & Wendong Jia & Jinyue Zhang & Xiumei Xu & Shuanghong Yan & Yuqin Wang & Panke Zhang & Hong-Yuan Chen & Shuo Huang, 2019.
"Giant single molecule chemistry events observed from a tetrachloroaurate(III) embedded Mycobacterium smegmatis porin A nanopore,"
Nature Communications, Nature, vol. 10(1), pages 1-11, December.
Handle:
RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13677-2
DOI: 10.1038/s41467-019-13677-2
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Citations
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Cited by:
- Pingping Fan & Shanyu Zhang & Yuqin Wang & Tian Li & Hanhan Zhang & Panke Zhang & Shuo Huang, 2024.
"Nanopore analysis of salvianolic acids in herbal medicines,"
Nature Communications, Nature, vol. 15(1), pages 1-12, December.
- Pingping Fan & Zhenyuan Cao & Shanyu Zhang & Yuqin Wang & Yunqi Xiao & Wendong Jia & Panke Zhang & Shuo Huang, 2024.
"Nanopore analysis of cis-diols in fruits,"
Nature Communications, Nature, vol. 15(1), pages 1-15, December.
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