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Cytokinetic abscission requires actin-dependent microtubule severing

Author

Listed:
  • Tamara Advedissian

    (Université Paris Cité, CNRS UMR3691, Membrane Traffic and Cell Division Unit)

  • Stéphane Frémont

    (Université Paris Cité, CNRS UMR3691, Membrane Traffic and Cell Division Unit)

  • Arnaud Echard

    (Université Paris Cité, CNRS UMR3691, Membrane Traffic and Cell Division Unit)

Abstract

Cell division is completed by the abscission of the intercellular bridge connecting the daughter cells. Abscission requires the polymerization of an ESCRT-III cone close to the midbody to both recruit the microtubule severing enzyme spastin and scission the plasma membrane. Here, we found that the microtubule and the membrane cuts are two separate events that are regulated differently. Using HeLa cells, we uncovered that the F-actin disassembling protein Cofilin-1 controls the disappearance of a transient pool of branched F-actin which is precisely assembled at the tip of the ESCRT-III cone shortly before the microtubule cut. Functionally, Cofilin-1 and Arp2/3-mediated branched F-actin favor abscission by promoting local severing of the microtubules but do not participate later in the membrane scission event. Mechanistically, we propose that branched F-actin functions as a physical barrier that limits ESCRT-III cone elongation and thereby favors stable spastin recruitment. Our work thus reveals that F-actin controls the timely and local disassembly of microtubules required for cytokinetic abscission.

Suggested Citation

  • Tamara Advedissian & Stéphane Frémont & Arnaud Echard, 2024. "Cytokinetic abscission requires actin-dependent microtubule severing," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46062-9
    DOI: 10.1038/s41467-024-46062-9
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    References listed on IDEAS

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    1. Luisa Capalbo & Zuni I. Bassi & Marco Geymonat & Sofia Todesca & Liviu Copoiu & Anton J. Enright & Giuliano Callaini & Maria Giovanna Riparbelli & Lu Yu & Jyoti S. Choudhary & Enrico Ferrero & Sally W, 2019. "The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    2. Silvia Arber & Freda A. Barbayannis & Hartwig Hanser & Corinna Schneider & Clement A. Stanyon & Ora Bernard & Pico Caroni, 1998. "Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase," Nature, Nature, vol. 393(6687), pages 805-809, June.
    3. Stéphane Frémont & Hussein Hammich & Jian Bai & Hugo Wioland & Kerstin Klinkert & Murielle Rocancourt & Carlos Kikuti & David Stroebel & Guillaume Romet-Lemonne & Olena Pylypenko & Anne Houdusse & Arn, 2017. "Oxidation of F-actin controls the terminal steps of cytokinesis," Nature Communications, Nature, vol. 8(1), pages 1-16, April.
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