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Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection

Author

Listed:
  • Eunyoung Jeon

    (Hanyang University
    Hanyang University
    Hanyang University)

  • Bonhan Koo

    (Yonsei University)

  • Suyeon Kim

    (Hanyang University
    Hanyang University
    Hanyang University)

  • Jieun Kim

    (Hanyang University)

  • Yeonuk Yu

    (Hanyang University)

  • Hyowon Jang

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Minju Lee

    (Yonsei University)

  • Sung-Han Kim

    (University of Ulsan College of Medicine)

  • Taejoon Kang

    (Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Sang Kyung Kim

    (Korea Institute of Science and Technology (KIST))

  • Rhokyun Kwak

    (Hanyang University)

  • Yong Shin

    (Yonsei University)

  • Joonseok Lee

    (Hanyang University
    Hanyang University
    Hanyang University)

Abstract

Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.

Suggested Citation

  • Eunyoung Jeon & Bonhan Koo & Suyeon Kim & Jieun Kim & Yeonuk Yu & Hyowon Jang & Minju Lee & Sung-Han Kim & Taejoon Kang & Sang Kyung Kim & Rhokyun Kwak & Yong Shin & Joonseok Lee, 2024. "Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45467-w
    DOI: 10.1038/s41467-024-45467-w
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    References listed on IDEAS

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    1. Seong Jun Park & Seungmin Lee & Dongtak Lee & Na Eun Lee & Jeong Soo Park & Ji Hye Hong & Jae Won Jang & Hyunji Kim & Seokbeom Roh & Gyudo Lee & Dongho Lee & Sung-Yeon Cho & Chulmin Park & Dong-Gun Le, 2023. "PCR-like performance of rapid test with permselective tunable nanotrap," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Ankur Deep Bordoloi & David Scheidweiler & Marco Dentz & Mohammed Bouabdellaoui & Marco Abbarchi & Pietro de Anna, 2022. "Structure induced laminar vortices control anomalous dispersion in porous media," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
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