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PCR-like performance of rapid test with permselective tunable nanotrap

Author

Listed:
  • Seong Jun Park

    (Kwangwoon University)

  • Seungmin Lee

    (Kwangwoon University
    Korea University)

  • Dongtak Lee

    (Korea University)

  • Na Eun Lee

    (Kwangwoon University
    Korea University)

  • Jeong Soo Park

    (Kwangwoon University)

  • Ji Hye Hong

    (Kwangwoon University
    Korea University)

  • Jae Won Jang

    (Korea University
    Korea University)

  • Hyunji Kim

    (Korea University
    Korea University)

  • Seokbeom Roh

    (Korea University
    Korea University)

  • Gyudo Lee

    (Korea University
    Korea University)

  • Dongho Lee

    (CALTH Inc.)

  • Sung-Yeon Cho

    (The Catholic University of Korea
    The Catholic University of Korea)

  • Chulmin Park

    (The Catholic University of Korea)

  • Dong-Gun Lee

    (The Catholic University of Korea
    The Catholic University of Korea)

  • Raeseok Lee

    (The Catholic University of Korea
    The Catholic University of Korea)

  • Dukhee Nho

    (The Catholic University of Korea
    The Catholic University of Korea)

  • Dae Sung Yoon

    (Korea University
    Korea University
    Astrion Inc)

  • Yong Kyoung Yoo

    (Catholic Kwandong University)

  • Jeong Hoon Lee

    (Kwangwoon University)

Abstract

Highly sensitive rapid testing for COVID-19 is essential for minimizing virus transmission, especially before the onset of symptoms and in asymptomatic cases. Here, we report bioengineered enrichment tools for lateral flow assays (LFAs) with enhanced sensitivity and specificity (BEETLES2), achieving enrichment of SARS-CoV-2 viruses, nucleocapsid (N) proteins and immunoglobulin G (IgG) with 3-minute operation. The limit of detection is improved up to 20-fold. We apply this method to clinical samples, including 83% with either intermediate (35%) or low viral loads (48%), collected from 62 individuals (n = 42 for positive and n = 20 for healthy controls). We observe diagnostic sensitivity, specificity, and accuracy of 88.1%, 100%, and 91.9%, respectively, compared with commercial LFAs alone achieving 14.29%, 100%, and 41.94%, respectively. BEETLES2, with permselectivity and tunability, can enrich the SARS-CoV-2 virus, N proteins, and IgG in the nasopharyngeal/oropharyngeal swab, saliva, and blood serum, enabling reliable and sensitive point-of-care testing, facilitating fast early diagnosis.

Suggested Citation

  • Seong Jun Park & Seungmin Lee & Dongtak Lee & Na Eun Lee & Jeong Soo Park & Ji Hye Hong & Jae Won Jang & Hyunji Kim & Seokbeom Roh & Gyudo Lee & Dongho Lee & Sung-Yeon Cho & Chulmin Park & Dong-Gun Le, 2023. "PCR-like performance of rapid test with permselective tunable nanotrap," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37018-6
    DOI: 10.1038/s41467-023-37018-6
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    Cited by:

    1. Eunyoung Jeon & Bonhan Koo & Suyeon Kim & Jieun Kim & Yeonuk Yu & Hyowon Jang & Minju Lee & Sung-Han Kim & Taejoon Kang & Sang Kyung Kim & Rhokyun Kwak & Yong Shin & Joonseok Lee, 2024. "Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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